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Figure 2 | Mobile DNA

Figure 2

From: LINE-1 and Alu retrotransposition exhibit clonal variation

Figure 2

Differences in LINE-1 retrotransposition between cell clones are maintained upon cell subcloning. (A) HeLa clones 1 and 7 were re-cloned and LINE-1 retrotransposition assays were performed. The differences in LINE-1 retrotransposition potential were maintained between HeLa subclones, with the four HeLa clone 1 subclones exhibiting a large reduction in LINE-1 retrotransposition rates compared to the four HeLa clone 7 subclones. (B) HCT116 clones 5 and 6 were re-cloned and LINE-1 retrotransposition assays were performed. As with the original HCT116 cell clones, there were no significant differences in LINE-1 retrotransposition rates between HCT116 subclones. Error bars represent SEM. Sample size is four independent experiments (independent transfections) each performed in triplicate (n = 12). Asterisks indicate statistical significance from the parental population of at least P <0.05 by one-way ANOVA with Tukey’s post-test. LINE-1, long interspersed element-1; SEM, standard error of the mean.

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