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Figure 1 | Mobile DNA

Figure 1

From: LINE-1 and Alu retrotransposition exhibit clonal variation

Figure 1

LINE-1 and Alu exhibit clonal variation. (A) LINE-1 retrotransposition assays were performed in eight individual HeLa cell clones and parental HeLa population. Different HeLa clones exhibit different potential for LINE-1 retrotransposition, with HeLa clone 1 exhibiting a 140-fold reduction in LINE-1 retrotransposition rate compared to HeLa clone 7. (B) Alu retrotransposition assays were performed in HeLa cell clones and parental HeLa. Differences in Alu retrotransposition between the two HeLa clones were even greater than those observed for LINE-1, with a 252-fold reduction in Alu retrotransposition in HeLa clone 1 compared to HeLa clone 7. (C) LINE-1 retrotransposition assays were also preformed in HCT116 cell clones to examine whether the effect seen in HeLa also occurred in HCT116 cells. The LINE-1 retrotransposition clonal variation was not observed in five HCT116 cell clones. (D) Alu retrotransposition assays were tested in seven HCT116 cell clones and parental HCT116 cell population and no significant differences in rates of Alu retrotransposition were observed between HCT116 clones. Error bars represent SEM. Sample size is four independent experiments (independent transfections) each performed in triplicate (n = 12). Asterisks indicate statistical significance from the parental population of at least P <0.05 by one-way ANOVA with Tukey’s post-test. LINE-1, long interspersed element-1; SEM, standard error of the mean.

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