Figure 7
From: Regulation of DNA transposition by CpG methylation and chromatin structure in human cells
Protamine enhances SB transposition. Transpositionally competent as well as incompetent (negative control) SB transposon plasmids were precomplexed with protamine in vitro, followed by transfection into transgenic HeLa cells stably expressing the SB transposase. The transfected cells have undergone antibiotic selection to allow a quantitative measurement of transposition efficiencies represented by antibiotic-resistant colony numbers. SB: Sleeping Beauty.