Figure 1

NucBase GUI. The NucBase graphical interface allows the user to load a file containing reads (a) which can be converted beforehand to the appropriate format (b). Then, a folder or a file containing the sequences of interest must be selected (c). It is also possible to directly copy and paste a sequence in the main window. Before starting the software, the user can specify several options to generate a global summary table giving the total number of matches for each read (d), to export the unmatched sequences (e), or to output only non-matching reads (f).