Identification of basepairs within Tn5 termini that are critical sfor H-NS binding to the transpososome and regulation of Tn5 transposition

Table 1 Oligonucleotides used in this study^a.

Name	Sequence (5' to 3')
CW1	CGCGTTTAATCTGCAGCACAGTCGTGATGGC
CW2	CCCTGCGCAGCGCGCAGCTGCAGCCTGAATACGCG
CW3	CTCGAC TGTCTCTTATACACATCTAGCGTCCTGAACGGAACCTTCTGCA
CW4	GAAGGTTCCGTTCAGGACGCTAGATGTGTATAAGAGACAG TCGAGGTAC
CW5	CTCGAC TGACTCTTATACACAAG TAGCGTCCTGAACGGAACCTTCTGCA
CW6	GAAGGTTCCGTTCAGGACGCTACTTGTGTATAAGAGT CAG TCGAGGTAC
CW7	CTCGAC TC TCTCTTATACACATCTAGCGTCCTGAACGGAACCTTCTGCA
CW8	GAAGGTTCCGTTCAGGACGCTAGATGTGTATAAGAGAG AG TCGAGGTAC
CW9	CTCGAC TGTCTCGC ATACACATCTAGCGTCCTGAACGGAACCTTCTGCA
CW10	GAAGGTTCCGTTCAGGACGCTAGATGTGTATGC GAGACAG TCGAGGTAC
CW11	NNNAAGCTTGGGTAACGCCAGGGTTTTCCCACTC
CW12	NNNTCTAGACGCCAAGCTTGCATGCCTGCAGGTC
WT ME 53NTS	CCCTGCAGGTCGAC TGTCTCTTATACACATCTTGAGTGAGTGAGCATGCA
WT ME 53TS	ACATGCATGCTCACTCACTCAAGATGTGTATAAGAGACAG TCGACCTGCAGGG
OE 53NTS	CCCTGCAGGTCGAC TGACTCTTATACACAAG TTGAGTGAGTGAGCATGCA
OE 53TS	ACATGCATGCTCACTCACTCAACTTGTGTATAAGAGT CAG TCGACCTGCAGGG
ME 3 53NTS	CCCTGCAGGTCGAC TC TCTCTTATACACATCTTGAGTGAGTGAGCATGCA
ME 3 53TS	ACATGCATGCTCACTCACTCAAGATGTGTATAAGAGACAG TCGACCTGCAGGG
ME 8/9 53NTS	CCCTGCAGGTCGAC TGTCTCGC ATACACATCTTGAGTGAGTGAGCATGCA
ME 8/9 53TS	ACATGCATGCTCACTCACTCAAGATGTGTATGC GAGACAG TCGACCTGCAGGG
T1TaseF	GACCTCTTAAGATGGTAACGTTCATG
T1TaseR	GCCGAAGAGAACACAGATTTAGC
T1TaseProbe^b	6FAM-TAACTTCTGCTCTTCATCGTG-MGBNFQ
16SF	ACCAGGGCTACACACGTGCTA
16SR	TCTCGCGAGGTCGCTTCT
16SProbe^b	6FAM-AATGGCGCATACAAA-MGBNFQ

^aThe first nucleotide of the transposon end sequence (1 or +1 position) of each strand is in bold. The mutated nucleotides are underlined. ^b6FAM represents 6-carboxyfluorescein. MGBNFQ represents the quencher dye.

ISSN: 1759-8753