Skip to main content
Figure 4 | Mobile DNA

Figure 4

From: Identification of basepairs within Tn5 termini that are critical sfor H-NS binding to the transpososome and regulation of Tn5 transposition

Figure 4

Mass spectrometry of cross-linked product 'a'. (A) Coomassie blue-stained SDS gel containing gel-purified, EDC/NHS cross-linked H-NS-transpososome (lane 2), purified transposase (lane 3) and purified H-NS (lane 4). Plugs of acrylamide from the boxed areas including transposase, H-NS and product 'a' were excised using a 'spot-picker', digested with trypsin, and analyzed by MALDI-TOF. (B) Two sets of spectra derived from transposase monomer (m), H-NS monomer and 'a' bands are shown. In both sets the spectra from 'a' have peptides that are also found in the spectra derived from transposase and H-NS. Peaks are separated on the x-axis based on mass/charge ratio. *, peptides corresponding to transposase;filled triangle, peptides corresponding to H-NS. MALDI-TOF, matrix-assisted laser desorption/ionization-time of flight.

Back to article page

Mobile DNA

ISSN: 1759-8753