(A) Plot of Ty1 insertions upstream of tRNAs. The blue curve above the midline represents Ty1 insertions in tandem with the tRNAs, that below the midline represents elements inserted in inverted orientation. (B) Scheme of Ty1 integration in nucleosomal DNA. The black lines represent nucleosomal DNA from base 1 to base 146 (5' to 3' orientation), the red boxes are the hotspots of integration with the coordinates of the attacked dinucleotides indicated above. The green broken lines indicate the expected integration hotspots symmetrically disposed around the dyad axis and distant of 73 bp. We can see here the "right shift" of the observed integration hotspots towards the tRNA gene in regard to the dyad axis of symmetry.