Figure 2

Shapiro model for transposition of Mu and other elements. Taken from [12]. The original legend for this figure reads as follows: “Transposition and replication. The top cartoon illustrates how various DNA regions may be brought into close physical proximity for the subsequent cleavage and ligation events. The bottom four drawings show various steps in the transposition process as described in the text. Solid lines indicate donor DNA; dashed lines indicate target DNA; the heavy bars are parental DNA of the transposable element, and open bars are newly synthesized DNA; the small boxes indicate the oligonucleotide target sequence (filled, parental DNA; open, newly synthesized DNA). The arrowheads indicate 3’-hydroxyl ends of DNA chains and the dots indicate 5’-phosphate ends. The letters a, b, c and d in the duplex arms flanking the transposable elements and target oligonucleotide serve to indicate the genetic structure of the various duplex products.” This figure is reproduced with permission from James Shapiro.