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Table 1 RHFs identified as Ty1 or Ty3 RHFs in earlier genetic screens

From: Host co-factors of the retrovirus-like transposon Ty1

RHF

Systematic gene name

Cellular function

Reference

Relative Ty1 cDNA level (rhfΔ/RHF)

Apq12

YIL040W

Protein required for nuclear envelope morphology, nuclear pore complex localization, mRNA export from the nucleus; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism

[14]

1.39

Bro1

YPL084W

Class E vacuolar protein sorting factor that coordinates deubiquitination in the multivesicular body (MVB) pathway

[16]

1.54

Ccr4

YAL021C

Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening

[23]

0.65

Cdc50

YCR094W

Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity

[14]

0.38

Cpr7

YJR032W

Peptidyl-prolyl cis-trans isomerase, Hsp90 co-chaperone

[10, 16]

0.67

Dhh1

YDL160C

Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping

[14, 16]

0.23

Elp2

YGR200C

Subunit of transcriptional elongator complex (HAT)

[10]

0.21

Glo2

YDR272W

Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate

[14]

1.04

Hda3

YPR179C

Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex

[14]

1.84

Hmo1

YDR174W

Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5′-3′ DNA helicase and Fpr1p, a prolyl isomerase

[14]

0.19

Ksp1

YHR082C

Nuclear serine/threonine kinase; stress response

[15]

1.79

Loc1

YFR001W

Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66 S pre-ribosomal particles

[14]

0.14

Lsm1

YJL124C

Component of heteroheptameric complex involved in cytoplasmic mRNA degradation

[10, 14]

0.53

Met5

YJR137C

Sulfite reductase, involved in amino acid biosynthesis and transcription repressed by methionine

[15]

1.04

Mig3

YER028C

Probable transcriptional repressor involved in response to toxic agents that inhibit ribonucleotide reductase; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes

[14]

1.75

Ncl1

YBL024W

tRNA:m5C-methyltransferase

[15]

0.49

Nip100

YPL174C

Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150 (glued)

[14]

1.06

Nup133

YKR082W

Subunit of the Nup84p subcomplex of the nuclear pore complex

[10]

3.13

Nup170

YBL079W

Subunit of the nuclear pore complex (NPC), required for NPC localization of specific nucleoporins; involved in nuclear envelope permeability and chromosome segregation; has similar to Nup157; essential role, with Nup157, in NPC assembly

[14]

0.66

Oca4

YCR095C

Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae

[14]

0.43

Pde2

YOR360C

High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP

[14]

0.92

Ref2

YDR195W

RNA-binding protein involved in the cleavage step of mRNA 3′-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3′-ends of snoRNA- and mRNA-encoding genes

[14]

0.17

Rpl16B

YNL069C

Component of the large (60 S) ribosomal subunit; binds to 5.8 S rRNA; has similarity to Rpl16A

[10]

0.51

Rpl27a

YHR010W

Protein component of the large (60 S) ribosomal subunit, nearly identical to Rpl27B

[14]

0.19

Rpp1A

YDL081C

Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome

[10]

1.23

Rps19b

YNL302C

Protein component of the small (40 S) ribosomal subunit, required for assembly and maturation of pre-40 S particles; mutations in human RPS19 are associated with Diamond Blackfan anemia; nearly identical to Rps19A

[14]

0.28

Rps25a

YGR027C

Protein component of the small (40 S) ribosomal subunit; nearly identical to Rps25B

[14]

0.19

Ski8

YGL213C

Ski complex component and WD-repeat protein, mediates 3′-5′ RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination

[14]

0.58

Snf5

YBR289W

Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p

[32]

0.09

Snf6

YHL025W

Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p

[32]

0.27

Spt3

YDR392W

Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes

[33]

0.09

Spt8

YLR055C

Subunit of the SAGA transcriptional regulatory complex

[14]

0.10

Spt10

YJL127C

Putative histone acetylase; sequence-specific activator of histone genes

[10, 14]

1.17

Sqs1

YNL224C

Stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18 s rRNA maturation by Nob1p; component of pre-ribosomal particles

[14]

0.63

Sse1

YPL106C

ATPase; Hsp90 co-chaperone; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family

[10]

0.25

Swi3

YJL176C

Subunit of the SWI/SNF chromatin remodeling complex

[14]

0.44

Tgs1

YPL157W

Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for ribosome synthesis and nucleolar morphology

[14]

1.44

Thp2

YHR167W

Subunit of the THO/TREX complex, couples transcription to mRNA export

[10]

0.98

Trk1

YJL129C

Component of the Trk1p-Trk2p high-affinity potassium transport system; plasma membrane protein

[10, 14]

1.14

Ump1

YBR173C

Chaperone required for correct maturation of the 20 S proteasome

[14]

0.36

Upf1

YMR080C

ATP-dependent RNA helicase of the SFI superfamily involved in nonsense-mediated mRNA decay (NMD); required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance

[14, 23]

0.25

Upf3

YGR072W

Component of the NMD pathway, along with Nam7 and Nmd2/Upf2; involved in decay of mRNA containing nonsense codons

[14, 23]

0.29

Vma16

YHR026W

Subunit c of vacuolar-ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain

[15]

1.32

Vph1

YOR270C

Subunit a of vacuolar-ATPase, V0 domain which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain

[10, 16]

1.55

YML009C-A

YML009C-A

Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; ORF overlaps the essential gene, SPT5

[14]

N.D.