RHF | Systematic gene name | Cellular function | Reference | Relative Ty1 cDNA level (rhfΔ/RHF) |
---|---|---|---|---|
Apq12 | YIL040W | Protein required for nuclear envelope morphology, nuclear pore complex localization, mRNA export from the nucleus; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism | [14] | 1.39 |
Bro1 | YPL084W | Class E vacuolar protein sorting factor that coordinates deubiquitination in the multivesicular body (MVB) pathway | [16] | 1.54 |
Ccr4 | YAL021C | Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening | [23] | 0.65 |
Cdc50 | YCR094W | Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity | [14] | 0.38 |
Cpr7 | YJR032W | Peptidyl-prolyl cis-trans isomerase, Hsp90 co-chaperone | 0.67 | |
Dhh1 | YDL160C | Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping | 0.23 | |
Elp2 | YGR200C | Subunit of transcriptional elongator complex (HAT) | [10] | 0.21 |
Glo2 | YDR272W | Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate | [14] | 1.04 |
Hda3 | YPR179C | Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex | [14] | 1.84 |
Hmo1 | YDR174W | Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5′-3′ DNA helicase and Fpr1p, a prolyl isomerase | [14] | 0.19 |
Ksp1 | YHR082C | Nuclear serine/threonine kinase; stress response | [15] | 1.79 |
Loc1 | YFR001W | Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66 S pre-ribosomal particles | [14] | 0.14 |
Lsm1 | YJL124C | Component of heteroheptameric complex involved in cytoplasmic mRNA degradation | 0.53 | |
Met5 | YJR137C | Sulfite reductase, involved in amino acid biosynthesis and transcription repressed by methionine | [15] | 1.04 |
Mig3 | YER028C | Probable transcriptional repressor involved in response to toxic agents that inhibit ribonucleotide reductase; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes | [14] | 1.75 |
Ncl1 | YBL024W | tRNA:m5C-methyltransferase | [15] | 0.49 |
Nip100 | YPL174C | Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150 (glued) | [14] | 1.06 |
Nup133 | YKR082W | Subunit of the Nup84p subcomplex of the nuclear pore complex | [10] | 3.13 |
Nup170 | YBL079W | Subunit of the nuclear pore complex (NPC), required for NPC localization of specific nucleoporins; involved in nuclear envelope permeability and chromosome segregation; has similar to Nup157; essential role, with Nup157, in NPC assembly | [14] | 0.66 |
Oca4 | YCR095C | Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae | [14] | 0.43 |
Pde2 | YOR360C | High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP | [14] | 0.92 |
Ref2 | YDR195W | RNA-binding protein involved in the cleavage step of mRNA 3′-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3′-ends of snoRNA- and mRNA-encoding genes | [14] | 0.17 |
Rpl16B | YNL069C | Component of the large (60 S) ribosomal subunit; binds to 5.8 S rRNA; has similarity to Rpl16A | [10] | 0.51 |
Rpl27a | YHR010W | Protein component of the large (60 S) ribosomal subunit, nearly identical to Rpl27B | [14] | 0.19 |
Rpp1A | YDL081C | Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome | [10] | 1.23 |
Rps19b | YNL302C | Protein component of the small (40 S) ribosomal subunit, required for assembly and maturation of pre-40 S particles; mutations in human RPS19 are associated with Diamond Blackfan anemia; nearly identical to Rps19A | [14] | 0.28 |
Rps25a | YGR027C | Protein component of the small (40 S) ribosomal subunit; nearly identical to Rps25B | [14] | 0.19 |
Ski8 | YGL213C | Ski complex component and WD-repeat protein, mediates 3′-5′ RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination | [14] | 0.58 |
Snf5 | YBR289W | Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p | [32] | 0.09 |
Snf6 | YHL025W | Subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p | [32] | 0.27 |
Spt3 | YDR392W | Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes | [33] | 0.09 |
Spt8 | YLR055C | Subunit of the SAGA transcriptional regulatory complex | [14] | 0.10 |
Spt10 | YJL127C | Putative histone acetylase; sequence-specific activator of histone genes | 1.17 | |
Sqs1 | YNL224C | Stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18 s rRNA maturation by Nob1p; component of pre-ribosomal particles | [14] | 0.63 |
Sse1 | YPL106C | ATPase; Hsp90 co-chaperone; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family | [10] | 0.25 |
Swi3 | YJL176C | Subunit of the SWI/SNF chromatin remodeling complex | [14] | 0.44 |
Tgs1 | YPL157W | Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for ribosome synthesis and nucleolar morphology | [14] | 1.44 |
Thp2 | YHR167W | Subunit of the THO/TREX complex, couples transcription to mRNA export | [10] | 0.98 |
Trk1 | YJL129C | Component of the Trk1p-Trk2p high-affinity potassium transport system; plasma membrane protein | 1.14 | |
Ump1 | YBR173C | Chaperone required for correct maturation of the 20 S proteasome | [14] | 0.36 |
Upf1 | YMR080C | ATP-dependent RNA helicase of the SFI superfamily involved in nonsense-mediated mRNA decay (NMD); required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance | 0.25 | |
Upf3 | YGR072W | Component of the NMD pathway, along with Nam7 and Nmd2/Upf2; involved in decay of mRNA containing nonsense codons | 0.29 | |
Vma16 | YHR026W | Subunit c of vacuolar-ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain | [15] | 1.32 |
Vph1 | YOR270C | Subunit a of vacuolar-ATPase, V0 domain which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain | 1.55 | |
YML009C-A | YML009C-A | Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; ORF overlaps the essential gene, SPT5 | [14] | N.D. |