Figure 2

Reproducibility of results of Ty1 his3AI retrotransposition assay across 10 trials. Progeny of 94 orfΔ::kanMX strains on one plate and the rtt101Δ query strain were isolated 10 independent times, and retrotransposition was measured in all 940 isolates. The fraction of trials at each address that yielded a ≥5-fold reduction in His⁺ papillae formation relative to the rtt101Δ query strain is plotted on the x-axis. The percentage of addresses within each category is plotted on the y-axis.