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Figure 9 | Mobile DNA

Figure 9

From: Protein-DNA interactions define the mechanistic aspects of circle formation and insertion reactions in IS2 transposition

Figure 9

Comparisons of protection patterns of the bottom strands of the single-end, MJcj and MJpc substrates. The sequence shown is that of the bottom strand of the MCJ with the spacer base guanine separating the abutted right and left ends. Numbering of the bases is as described in Figure 7. Protection patterns are indicated by horizontal bars. Asterisks identify enhanced residues. Three stacked asterisks describe increased enhancement. Broken vertical lines within the large brackets demarcate the IRR and IRL cleavage domains (CD) and protein binding domains (PBD). Data for residues L30 to L42 and R32 to R41 of the MCJ substrates were difficult to interpret and are not shown. CD: cleavage domain; IRR/IRL: right and left inverted repeats; MCJ: minicircle junction; MJcj: covalently joined minicircle junction substrate; MJpc: precleaved minicircle junction substrate; PBD: protein binding domain.

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ISSN: 1759-8753