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Figure 5 | Mobile DNA

Figure 5

From: Protein-DNA interactions define the mechanistic aspects of circle formation and insertion reactions in IS2 transposition

Figure 5

Hydroxyl radical footprinting of the top (IRLA) and bottom (IRLB) strands of the IS 2 IRL. (A) Quantitative analysis panel showing relative intensities of bands from the footprint of IRLA (red) and the control DNA (green) as described in Figure 4. Determination of the protection profiles is as described in Figure 4. Bands and peaks are numbered (1 to 42) from the outer end of the terminus of IRR (the 5' end of the strand) to the inner end. The sequence of the top strand of IRL is shown below the panel. The IRL sequence (within large brackets and numbered as described above) is flanked by host DNA at the outer end of the terminus (-1 to -11) and the sequence of IS2 adjacent to the inner end of the terminus (43 to 50). (B) Quantitative analysis panel showing relative intensities of bands from the footprint of IRLB (red) and the control DNA (green). The zone of compression which masks the footprinting pattern from G5 to A-9 is shown more clearly in the inset. IRL: left inverted repeat.

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