Figure 6From: DNA binding activities of the Herves transposase from the mosquito Anopheles gambiaeThe CGATTCAT binding motif is sufficient for transposase binding. The probe (CGATTCAT)4 represents four direct repeats of the CGATTCAT sequence motif. Unlabeled (CGATTCAT)4 and Herves-left (L) bp 28-60 were used as specific competitors, whereas β2 tubulin was used as the non-specific competitor. The transposase binding was compared between the sequence variants of the binding motif such as CGATTCTT, CGATTCAC and CGTTCAT (each used as four direct repeats). The asterisk (*) indicates various protein DNA complexes.Back to article page