Skip to main content
Figure 1 | Mobile DNA

Figure 1

From: DNA binding activities of the Herves transposase from the mosquito Anopheles gambiae

Figure 1

Herves transposase binds to the terminal sequences of L and R ends of the Herves element. (a) Schematic representation of the Herves element. Numbers indicate the distance in bp internal to either the left (L) or right (R) end. (b) SDS-PAGE analysis of purified Herves transposase. A Coomassie-stained gel shows 70 kDa purified Herves transposase. E1-E5 represent different elutions obtained during the final step of protein purification. (c) Transposase binding to the terminal fragment of Herves-L and Herves-R ends. Electrophoretic mobility shift assay (EMSA) analysis with the Herves-L bp 1-100 (lanes 1-4) and Herves-R bp 1-100 (lanes 5-7) probes. The DNA fragments were incubated in the presence (+) or absence (-) of pure transposase. A homologous fragment was used as specific competitor. The E1 flanking sequence from the Hermes transposable element was used as the non-specific competitor [44]. Specific and non-specific competitors were use at 200-fold molar excess to the probe. Arrows indicate various protein DNA complexes.

Back to article page