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Figure 3 | Mobile DNA

Figure 3

From: Prevalence of SOS-mediated control of integron integrase expression as an adaptive trait of chromosomal and mobile integrons

Figure 3

Electrophoretic mobility-shift assay (EMSA) and quantitative real-time reverse transcription PCR on different intI genes and their respective promoters. (A) Vibrio parahaemolyticus integron. EMSA of V. parahaemolyticus intIA promoter with purified V. parahaemolyticus LexA protein. Competitive assays using either non-specific or P int non-labeled DNA are also shown. The intA expression factor was calculated as the ratio of the relative intA mRNA concentration in the V. parahaemolyticus lexA mutant strain with respect to that in the wild type. (B) E. coli pMUR050 integrons. EMSA of pMUR050 intI1-and intI1+(containing GGG insertion) promoters with purified E. coli LexA protein. The expression factor for both intI genes was calculated as the ratio of each relative intI mRNA concentration in the E. coli lexA-sulA mutant strain with respect to that in the wild type. In all cases, the expression factor of recA is shown as a control, and all expression factors are the mean value from three independent experiments (each in triplicate).

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