Table 2 Primers and probes used for quantitative real-time PCR.
From: Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers
Primer/probe name | Sequence 5'→3' |
|---|---|
RPPH1 | |
Forward | AGCTGAGTGCGTCCTGTCACT |
Reverse | TCTGGCCCTAGTCTCAGACCTT |
Probe | CACTCCCATGTCCC |
GFP | |
Forward | GAGCGCACCATCTTCTTCAAG |
Reverse | TGTCGCCCTCGAACTTCAC |
Probe | ACGACGGCAACTACA |
IRDR-L | |
Forward | CTCGTTTTTCAACTACTCCACAAATTTCT |
Reverse | GTGTCATGCACAAAGTAGATGTCCTA |
Probe | CTGACTTGCCAAAACT |
IRDR-R | |
Forward | GCTGAAATGAATCATTCTCTCTACTATTATTCTGA |
Reverse | AATTCCCTGTCTTAGGTCAGTTAGGA |
Probe | TCACCACTTTATTTTAAGAATGTG |