Real-time PCR assay designed for different transposon and transgene regions. (A) Structure of the used SB transposons with asymmetric IRDRs . For each construct, the TaqMan® assays (TQ) used for copy-number determination are indicated. Sequences are not drawn to scale. IRDR-L/-R = inverted repeat-direct repeat left/right regions; pA = SV40 polyadenylation signals. (B) Efficiencies of the real-time assays determined by standard curves. For all assays, a dilution series was prepared from pooled genomic DNA samples from clones containing integrated transposon 1. The efficiency of the IRDR-R TaqMan® assay was notably lower than that of the others (<90%).