Figure 5

Fluorescence in situ hybridization (FISH)-based visualization of the intrachromosomal distribution of chromoviruses. (A) Pea chromosomes hybridized with PiSat1 (group A). (B and C) Black cottonwood interphase nucleus and metaphase chromosomes hybridized with PopT2 (group A). Note that most of the signal is associated with chromocenters (bright 4',6-diamidino-2-phenylindole (DAPI)-stained spots in the interphase nucleus). Three metaphase chromosomes were enlarged to allow a clearer localization of PopT2 to the centromeric region (C1-C3). (D and E) White campion chromosomes hybridized with SilL1 and SilL2 (group A). (F) Banana chromosomes hybridized with MusA1 (group B). Since all of the banana chromosomes are metacentric or submetacentric, signals located around the center of the chromosome are taken to reflect loci near or within the centromere. Three of the chromosomes with identifiable centromeres were enlarged (F1-F3). (G and H) Norway spruce chromosomes counterstained with DAPI and hybridized with a Spdl-like sequence (group C). (I) Pea chromosomes hybridized with Peabody (Tekay clade). Positive hybridization signals are shown in green, and DAPI-counterstained DNA appears in red.