Skip to main content

Table 1 Results obtained from the qualitative excision tests

From: Effect of attC structure on cassette excision by integron integrases

Clone pLQ

Upstream att site

Cassette

Integron integrases (IntIs)

   

IntI1

IntI3

IntI2*179E

SonIntIA

VchIntIA

423

attI1

ant(3'')-Ia T-N6-G

++

-

-

-

-

424

attI2

dfrA1 A-N7-8-C

-

-

+

-

-

425

attI3

bla IMP-1 T-N6-G*

-

-

-

-

-

426

attC dfrA1 A-N7-8-C

ant(3'')-Ia T-N6-G

+

-

+

+

-

427

attI1 N/A

dfrA1 A-N7-8-C

-

-

-

-

-

428

attC ant(3'')-Ic T-N6-G

aac(6')-Ia-orfG + orfH T-N6-G

+++

+++

+++

+++

+++

429

attI1 N/A

ant(3'')-Ic T-N6-G

++

-

-

-

-

430

attC dfrA1 A-N7-8-C

sat2 T-N6-G

+

+

++

++

-

431

attC aac(6')-Ib TC-N6-G

bla oxa10 T-N6-G

-

-

-

-

-

437

attC ant(3'')-Ic T-N6-G

dfrA1 A-N7-8-C

-

-

+

-

-

438

attC ant(3'')-Ic T-N6-G

bla IMP-1 T-N6-G*

+++

+++

+++

+++

-

439

attI1 N/A

bla IMP-1 T-N6-G*

+

-

-

-

-

440

attI1 N/A

aac(6')-Ia-orfG + orfH T-N6-G

+++

-

-

-

-

441

attI2 N/A

aac(6')-Ia-orfG + orfH T-N6-G

++

-

+++

+

-

442

attI3 N/A

aac(6')-Ia-orfG + orfH T-N6-G

+

-

-

-

+

443

attC ant(3'')-Ic T-N6-G

ant(3'')-Ia T-N6-G

+++

+++

+++

+++

+++

444

attC dfrA1 A-N7-8-C

dfrA1 A-N7-8-C

+

+

+++

+++

-

445

attC aac(6')-Ia -orfG T-N6-G

ant(3'')-Ia T-N6-G

+++

+++

++

++

++

446

attC blaIMP-1 T-N6-G

aac(6')-Ia-orfG + orfH T-N6-G

+++

+++

+++

+++

-

  1. -, No excision; +, weak excision (<20%); ++, moderate excision (20 to 75%); +++, strong excision (>75%); *, additional secondary structure between the extrahelical bases.