Characterization of a synthetic human LINE-1 retrotransposon ORFeus-Hs

Table 2 The 3' untranslated region and constitutive transport element are not essential to ORFeus-Hs retrotransposition.

Construct^a	CMV^b+5" UTR^c	CMV+5" UTR+Kozak	CMV+Kozak
Fully native	1.0 ± 0.1 [pWA192]	ND^d	ND
ORFeus-Hs, no 3" UTR	1.7 ± 0.4 [pWA180]	1.6 ± 0.5 [pWA181]	1.9 ± 0.4 [pWA200]
ORFeus-Hs + 3(UTR	2.4 ± 0.7 [pWA184]	2.8 ± 1.2 [pWA185]	2.0 ± 0.7 [pWA182]
ORFeus-Hs +3" UTR + CTE^e	1.1 ± 0.4 [pWA199]	1.1 ± 0.6 [pWA186]	1.3 ± 0.2 [pWA183]

^aFor each construct, retrotransposition frequency is Retrotransposition frequencies are represented as fold changes relative to native L1_RP [pWA192]. The average number of G418R colonies wass 157 per 104 cells seeded. Data are the mean ± SE of six independent experiments. Plasmid names are in square brackets.
^bCMV = cytomegalovirus
^cUTR = untranslated region
^dND = not determined.
^eCTE = constitutive transport element

ISSN: 1759-8753