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Table 1 Retrotransposition frequency of ORFeus-Hs constructs.

From: Characterization of a synthetic human LINE-1 retrotransposon ORFeus-Hs

Constructa

CMV+Kozakb

CMV+ 5" UTRc

5" UTR

Fully native

2.8 ± 0.1 (1) [pLD223]

14 ± 0.3 (1) [pWA174]

3.8 ± 0.3 (1) [pLD143]

Chimera-P

5.8 ± 0.3 (2.07) [pWA172]

11.2 ± 0.2 (0.8) [pLD224]

5.1 ± 0.1 (1.34) [pLD252]

Chimera-E

8.8 ± 0.5 (3.14) [pWA170]

12.1 ± 0.3 (0.86) [pLD227]

5.4 ± 0.3 (1.42) [pLD253]

Chimera-B

10.8 ± 0.4 (3.85) [pWA176]

14.3 ± 0.6 (1.02) [pLD225]

6.9 ± 0.4 (1.82) [pLD254]

ORFeus-Hs

8.1 ± 0.3 (2.89) [pWA163]

8.1 ± 0.4 (0.58) [pWA165]

4.5 ± 0.2 (1.18) [pLD255]

ORFeus-Mm

NDd

9.2 ± 0.8 (0.66) [pWA125]

ND

  1. aFor each construct, its absolute retrotransposition frequency is represented as percentage of enhanced green fluorescence-positive cells, and data are the mean ± SEM of eight independent experiments. The number in the parentheses represents the fold change of retrotransposition frequency relative to native L1RP (shown in the first row), and the relevant plasmid name is given in square brackets.
  2. bCMV = cytomegalovirus
  3. cUTR = untranslated region.
  4. dND = not determined.