Figure 1
From: Characterization of a synthetic human LINE-1 retrotransposon ORFeus-Hs
Schematic representation of native, synthetic and chimeric human L1 elements. Three sets of such constructs differing from each other at the promoter region are illustrated: the first set carries a cytomegalovirus (CMV) promoter and a Kozak (K) signal, the second set has a dual CMV-L1 5' untranslated region (UTR) promoter, and the third has a 5' UTR promoter only. All elements are cloned in a pCEP-Puro vector backbone. AMPR = ampicillin resistance gene; B, E, P = restriction sites EcoR I, BamH I and Pml I, respectively, at the junctions in various chimeras; EBNA-1 = Epstein-Barr nuclear antigen 1 gene permitting extrachromosomal replication; Intron = human gamma globin intron; Marker = either enhanced green fluorescent protein or neomycin marker; ORF = open reading frame; PuroR = puromycin resistance gene; SV40pA = Simian virus 40 polyadenylation signal. Blue = native sequence; purple = synthetic sequence.