Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalis

Table 2 Integration site analysis for remobilized progeny from 7M Hoppers

Tadpole	Right IR/DR flanking sequence	Integration site	5' flanking gene	3' flanking gene
Parental 7M	TGTTAGTTATTACTTA cagttgaagtcgg	38:3796832	EDEM2	PHF20
E208-91	TCATTATCAGTATATA cagttgaagtcgg	38: 552620	EMILIN3	EMILIN3
3-1	GCTACTCACACAGTTA cagttgaagtcgg	13: 899336	SPRY2	NDFIP2
2-7M	TGTTACTGGGCACTTA cagttgaagtcgg	3: 3883685	MYNN	MDS1
A21A-1	AGTGTGTAGCTATATA cagttgaagtcgg	909:49452	GBP3	GBP3
C2AB-5	CGGGCCATGATGTATA cagttgaagtcgg	Repeat	-	-
E2F3-95	GCACATAATACACATcagttgaagtcgg	Unknown	-	-
E220-248	CATGTA cagttgaagtcgg	Unknown	-	-

Sequences flanking seven novel transposon insertion sites from 7M hoppers were cloned using extension primer tag selection linker mediated-PCR. The transposon sequence (italics) is flanked by the characteristic TA dinucleotide target site duplication (bold). The flanking sequence (depicted in capital letters) was used to interrogate the X. tropicalis genome sequence database (Joint Genome Institute X. tropicalis genome sequence assembly v4.1; [3]) to assign the integration sites to the genomic sequence scaffolds. The genes flanking the novel integration site are indicated.