Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalis

Table 1 Integration site analysis for remobilized progeny from 8F hoppers.

Tadpole	Right indirect repeat/direct repeats flanking sequence	Integration site	5' flanking gene	3' flanking gene
Parental 8F	GCAACGCTagtcac...cagttATTGATT^a	57:2456981	pSST739	C7orf72-like
8F♂51-498	GCGCAATACTATTATA cagttgaagtcgg	57:2452131	pSST739	C7orf72-like
8F♂51-55	CGGGCCATGATGTATA cagttgaagtcgg	pT2βGFP^b	pSST739	C7orf72-like
8F♀61-1	GTGGAGCTCTGAAATA cagttgaagtcgg	pT2βGFP^b	pSST739	C7orf72-like
8F♂58-2	AAAGGCAACACGCGTA cagttgaagtcgg	57:2470830	pSST739	C7orf72-like
8F♂58-8	GAATCTCTGTGATCTA cagttgaagtcgg	57:2491386	pSST739	C7orf72-like
8F♂C-43	CAGAGCTAGATATATA cagttgaagtcgg	57:2507804	C7orf72-like	C7orf72-like
8F35♀F-1	TGGAAATGCCTATATA cagttgaagtcgg	57:2544323	C7orf72-like	Ikaros
8F35♂C-44	AAGAAAGCACTTGGTA cagttgaagtcgg	57:2672369	Dopa decarboxylase	Dopa decarboxylase
8F♀60-1	CCCCCTTCGGTGATTA cagttgaagtcgg	57:2897938	Grb10	Cordon-bleu
8F35♂A-207	ACAAACGGGCCATGTA cagttgaagtcgg	57:2991209	Grb10	Cordon-bleu
8F♂58-9	TATCTAAACAAAGTTA cagttgaagtcgg	588:587502	Cam-PDE 1C	Cam-PDE 1C
6265	TCACTACATATTTCTA cagttgaagtcgg	294: 394261	PTPRM	PTPRM
8F♂58-3	TAAGAATTAATAGTTA cagttgaagtcgg	250:752306	c-Fyn	c-Fyn
8F35♂C-35	TATAAATAAAGATATA cagttgaagtcgg	223:803513	Connexin 31.1	C1orf94-like
8F♂B-203	AAGGCAGTCAGTTATA cagttgaagtcgg	15:2263789	RDC-1	COP9
8F35♂A-205	GATAACTCTTAAGTTA cagttgaagtcgg	484:822241	Amphiphysin	Amphiphysin

Sequences flanking the novel transposon insertion site were cloned using extension primer tag selection linker mediated-PCR. The remobilization events are mediated by canonical transposition reactions and the transposon sequence (italics) is flanked by the characteristic TA dinucleotide target site duplication (bold). The flanking sequence (depicted in capital letters) was used to interrogate the X. tropicalis genome sequence database (Joint Genome Institute X. tropicalis genome sequence assembly v4.1; [3]) to assign the integration sites to the genomic sequence scaffolds. The genes flanking the novel integration site are listed.
^aThe parental integration site on scaffold 57 was mediated by a non-canonical mechanism. ^bTwo remobilization events showed reintegration of the excised transposon into the donor concatamer locus on scaffold 57.

ISSN: 1759-8753