Figure 4

Chimpanzee-specific APE deletions. PCR analysis confirmed chimpanzee-specific APE deletions in orthologous human, chimpanzee, gorilla, orangutan and rhesus macaque loci. Human adjacent inverted FAP loci were chosen with spacer sizes between 651 and 1500 bp and a minimum of 1,000 bp of Alu-free flanking sequence. PCR loci were selected for which the chimpanzee loci were > 350 bp shorter than the human ortholog. Using identical primers, PCRs were then prepared for human, chimpanzee, gorilla, orangutan and rhesus macaque. APE: Alu pair exclusion; bp: base pair; FAP: full-length Alu pair; PCR: polymerase chain reaction.