Reverse transcription of the pFOXC mitochondrial retroplasmids of Fusarium oxysporum is protein primed

Table 3 Relative intensity of the 60 kDa nucleotide-linked protein in reactions having different combinations of deoxynucleotides and dideoxynucleotides

Radiolabeled nucleotide plus three dNTPs^a	Plus one ddNTP and two dNTPs				Plus three ddNTPs
	ddA	ddG	ddT	ddC
A*+CGT (1.0)	-	+CT (0.9)	+CG (1.2)	+GT (1.2)	ddCGT (0.2)
G*+ACT (1.0)	+CT (0.6)	-	+AC (1.6)	+AT (1.5)	ddACT (0.3)
T*+ACG (1.0)	+CG (0.4)	+AC (0.6)	-	+AG (0.9)	ddACG (0.3)
C*+AGT (1.0)^b	+GT (0.5)	+AT (1.2)	+AG (3.3)	-	ddAGT (0.3)

^aAsterisk indicates the radiolabeled nucleotide used in all reactions in the row and numbers in parentheses indicate the intensity of the 60 kDa nucleotide-linked protein relative to labeling intensity obtained in reactions having a single [α-³²P]dNTP plus three unlabeled dNTPs.
^bExperiments using [α-³²P] deoxycytidine triphosphate resulted in non-discrete products that complicated accurate quantification.
ddNTP = dideoxynucleotide triphosphates; dNTP = deoxynucleotide triphosphate.

ISSN: 1759-8753