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Table 2 Relative intensity of the 60 kDa nucleotide-linked protein in reactions having different combinations of deoxynucleotides

From: Reverse transcription of the pFOXC mitochondrial retroplasmids of Fusarium oxysporum is protein primed

Radiolabeled nucleotidea

Plus one unlabeled dNTP

Plus two unlabeled dNTPs

Plus three dNTPs

A* (1.0)

G (1.6) > C (1.3) > T (1.0)

CG (17.5) > GT (13.4) > CT (2.6)

CGT (6.3)

G* (1.0)

A (3.0) > C (0.9) > T (0.6)

AC (13.9) > AT (7.9) > CT (1.7)

ACT (4.3)

T* (1.0)

A (1.4) > C (1.3) > G (0.2)

AG (10.8) > AC (8.2) = CG (8.2)

ACG (5.7)

C* (1.0)b

A (1.7) > G (1.0) > T (0.6)

AG (2.4) > AT (1.5) > GT (0.7)

AGT (0.7)

  1. aAsterisk indicates the radiolabeled nucleotide used in all reactions in the row and numbers in parentheses indicate the intensity of the nucleotide-linked protein product relative to labeling intensity obtained in reactions having a single [α-32P]dNTP.
  2. bExperiments using [α-32P]deoxycytidine triphosphate resulted in non-discrete products that complicated accurate quantification.
  3. dNTP = deoxynucleotide triphosphate.