Figure 6

A nucleotide-linked protein generated with [α-³²P]thymidine triphosphate (TTP) in pFOXC1-mitochondrial ribonucleoproteins (mtRNPs) is chased into a protein-cDNA product. (a) Micrococcal nuclease (MN)-treated pFOXC1-containing mtRNPs incubated with [α-³²P]dATP or [α-³²P]TTP and a full complement of deoxynucleotides, with or without a 92 nucleotide RNA template, as indicated. (b) MN-treated pFOXC1-containing mtRNPs incubated with [α-³²P]TTP with or without a 92 nucleotide RNA corresponding to the 3' end of pFOXC1. All four unlabeled deoxyribonucleotide triphosphates (dNTPs) were added to reactions to a final concentration of 20 μM at the times indicated. All reactions were terminated with Laemmli buffer and reaction products were separated via 4-20% gradient SDS-PAGE. Prestained protein size markers are indicated on the left in kDa.