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Table 1 Diversity at coding joint sequences

From: Detecting variable (V), diversity (D) and joining (J) gene segment recombination using a two-colour fluorescence system

RSS23 coding end sequence Nucleotides lost Nucleotides added Nucleotides lost RSS12 coding end sequence
ATTACGCGC*     *GGTACCGTC
Deletion substrate:     
ATTACGCG -C +GC -G GTACCGTC
ATTACG -CGC   -GG TACCGTC
ATTACGC -GC   -GGTAC CGTC
ATTACGCG -C +GG -GGTA CCGTC
ATTACGCG -C   -GG TACCGTC
ATTACGCG -C +GCG -GGTA CCGTC
ATTACG -CGC   -G GTACCGTC
ATTACGCG -C   -G GTACCGTC
ATTACGCG -C   -G GTACCGTC
Inversion substrate:     
ATTACGCGC -C +G -GGTA CCGTC
ATTACG -CGC   -GG TACCGCG
ATTACGCG -C +GC -GGT ACCGTC
ATTACGCG -C    GGTACCGTC
ATTACGCG -C +GC -GGT ACCGTC
ATTACGCG -C +GC -GGT ACCGTC
ATTACG -CGC   -GG TACCGTC
ATTACGC -GC   -GGTAC CGTC
ATTACGCG -C +GC -G GTACCGTC
ATTACGCG -C   -G GTACCGTC
  1. DNA is cleaved at the boundary of the recombination signal sequence (RSS) motifs and the ends are joined. Joining involves the trimming of nucleotides and the addition of bases via terminal deoxynucleotide transferase (TdT), resulting in diversity at the coding joint.
  2. *The sequences marked indicate the predicted sequence if recombination involved no nucleotide loss or addition. The actual sequences obtained for the deletion and inversion substrate are shown, with nucleotides loss and gained indicated.