Figure 5

Fluorescence in situ hybridization (FISH) and differential staining with 4',6-diamidino-2-phenylindole (DAPI) on somatic and meiotic chromosomes of Aegilops speltoides (part 1). (a) FISH with 5S rDNA, 45S rDNA and staining with DAPI on somatic chromosomes of the original G9 plant (left). Chromosomes 1, 6 (arrows), and B chromosomes carry additional 5S rDNA sites (right). (b) FISH with Spelt 52, Spelt 1 (arrows on B chromosomes), 5S rDNA and 45S rDNA on the somatic chromosomes of the G9 S1 plant (left); FISH with 5S rDNA (right). Chromosomes 1 and 6 carry additional 5S rDNA sites (arrowed). (c) From left to right: FISH with 5S rDNA and 45S rDNA, and DAPI on the meiotic chromosomes of the G9 S2 plant; 5S rDNA probe alone, chromosomes 1, 6 (arrows), and B chromosomes carry additional 5S rDNA sites; FISH with Spelt 52 and Spelt 1 on the same chromosomes; FISH with CCS-1 and 45S rDNA (a pericentric inversion is arrowed); the scheme of the main chromosomal rearrangements (see legend). (d) DAPI (left) and FISH (middle) with 5S rDNA and 45S rDNA on the meiotic chromosomes of the G13 S2 plant. A scheme of the main chromosomal rearrangements (right). (e) FISH with Spelt 52 and Spelt 1 on the meiotic chromosomes of the G13 S3 plant (left). Small Spelt 52 cluster (arrow) marks paracentric inversion in the long arm of the chromosome 5. FISH with 5S rDNA and 45S rDNA with DAPI staining (middle). Both termini of chromosome 5 are involved in heterologous synapses (white arrows); heterozygous deletion on the chromosome 6 is shown by yellow arrow. Chromosomes 1 and 6 carry additional 5S rDNA clusters (arrows in right). The DNA probes and staining: (a-e) 5S rDNA, Spelt 52 and cereal centromere-specific sequence 1 (CCS-1) in red; 45S rDNA and Spelt 1 in green; differential staining with DAPI in blue; (b) 5S rDNA (yellow) and 45S rDNA (blue) in pseudocolors.