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Figure 1 | Mobile DNA

Figure 1

From: Transposition of the Tourist-MITE mPing in yeast: an assay that retains key features of catalysis by the class 2 PIF/Harbinger superfamily

Figure 1

Constructs used in this study and steps in the yeast assay. Diagram showing the relationship of the native Ping and mPing elements to the constructs used for the yeast transposition assay (A). ORF1 (green) and TPase (orange) coding regions were placed in galactose inducible constructs, while mPing (red) was cloned into the ADE2 coding region (yellow). The transposition assay protocol (B) involves growing transformed yeast in non-inducing liquid media and then plating on inducing (galactose) media under selection for adenine autotrophy. mPing excision and repair of ADE2 results in colony formation.

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