Figure 3

The effect of growth conditions on transposition frequency. (a) Western blot analysis of MuA expression levels at varying arabinose concentrations. Whole cell lysates from E. coli DH5α cells containing plasmid pLHH4 were analysed for MuA content using polyclonal rabbit anti-MuA antibody. The asterisk indicates MuA. (b) The effect of arabinose concentration on colony size and papillation. Wild-type MuA-expressing plasmid (pLHH4) was used for papillation analysis with E. coli DH5α as a plasmid recipient strain. Open and filled circles indicate the colony size and the number of papillae per colony, respectively. Papillae were enumerated from six colonies for each data point. The error bars indicate SD above and below the average value for each data point.