Figure 8
From: Regulators of ribonucleotide reductase inhibit Ty1 mobility in saccharomyces cerevisiae
Effect of hydroxyurea on Ty1 mobility. (A) Yeast strains containing the galactose inducible Ty1 element on plasmid pGTy1his3 AI[Δ 1] were induced at permissive temperature (28°C) in the presence of varying concentrations of HU and quantitatively assayed for pGTy1 mobility. HU treatment increases pGTy1 mobility in both wild type and sml1Δ strains, but not in the rfx1Δ strain. (B) pGTy1 mobility patch assay at 32 and 34°C. HU treatment drastically reduces pGTy1 mobility in all strains at elevated temperatures. See Additional file 3 for numerical values.