Figure 4
From: Regulators of ribonucleotide reductase inhibit Ty1 mobility in saccharomyces cerevisiae
Pol protein processing is not affected in mutant strains. Indicated yeast strains containing the galactose-inducible plasmid pGTy1his3 AI[Δ 1] were grown at 26°C or 34°C in galactose for 0, 6 or 12 hours. Cell extracts were separated on SDS-PAGE and immunoblotted with monoclonal antibody to integrase or Gadph as a loading control. The integrase product was detected at 26°C only. Although the rfx1 and sml1 deletion strains showed more efficient processing of integrase at 26°C, no processed integrase was detected in any strains at 34°C. Molecular weight markers (in kDa) are shown on the left. Strains are wild type JKc1356; rfx1Δ JKc1427; sml1Δ JKc1429.