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Figure 1 | Mobile DNA

Figure 1

From: Feedback inhibition of L1 and alu retrotransposition through altered double strand break repair kinetics

Figure 1

Inverse correlation between endogenous full length L1 mRNA and retrotransposition potential. (A) Northern blot of endogenous L1 mRNA (see text for method). FL1 or full length L1 mRNA, as determined by size is denoted by an arrow. L1 mRNA species previously characterized as truncated products caused by internal splicing or premature polyadenylation are indicated by a bracket [22, 23]. As a loading control, the same blot was probed for β-actin RNA. (B) Inverse correlation between endogenous L1 mRNA Levels and retrotransposition potential. Mobilization of a tagged L1, which confers neomycin resistance to a cell upon successful retrotransposition, as described previously, [12] and the relative amount of endogenous full length L1 mRNA was measured in triplicate for HCT116, HeLa and MCF7 cells, and the means and standard deviations plotted.

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ISSN: 1759-8753