Figure 3

Integration sites of mini-Tnt1s. (A) Genomic DNA from calli with putative transposition events was digested with the restriction enzyme Hae III. After intramolecular ligation of the Hae III fragments, two pairs of primers (indicated by the horizontal arrows) specific for the 5' or 3' long terminal repeats (LTRs), the neomycin phosphotransferase gene (NPTII), or the nopaline synthase (NOS) promoter. were used in separate inverse PCR assays. (B) Sequences flanking six mini-Tnt1 insertions are shown in lowercase letters (three from mini-Tnt1 and three from 35S-mini-Tnt1), and duplicated sequences at the insertion site in uppercase letters.