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Table 5 Yeast 2 hybrid assay shows that TnsC and TnsD can interact in vivo.

From: Characterization of the TnsD-attTn7 complex that promotes site-specific insertion of Tn7

Protein fragment β-galactosidase activity
Fos Jun 5.0
E2F1 RB 1.0
Empty vector
Gal4-AD
Empty vector
Gal4-DB
0.075
TnsC (1 to 555) wt TnsD (1 to 507) wt 1.0
TnsC (1 to 555) wt TnsD (1 to 436) 0.075
TnsC (1 to 555) wt TnsD (1 to 399) 4.5
TnsC (1 to 555) wt TnsD (1 to 375) 5.0
TnsC (1 to 555) wt TnsD (1 to 350) 4.5
TnsC (1 to 555) wt TnsD (1 to 309)a 4.5
TnsC (1 to 555) wt TnsD (1 to 293) 0.075
TnsC (1 to 555) wt TnsD (8 to 507) 0.075
TnsC (1 to 555) wt TnsD (12 to 507) 0.075
TnsC (1 to 555) wt TnsD (16 to 507) 0.075
TnsC (1 to 555) wt TnsD (22 to 507) 0.075
TnsC (1 to 555) wt TnsD (1 to 507) wt 1.0
TnsC (1 to 480) TnsD (1 to 507) wt 1.0
TnsC (1 to 384) TnsD (1 to 507) wt 1.0
TnsC (1 to 332) TnsD (1 to 507) wt 0.20
TnsC (1 to 293)a TnsD (1 to 507) wt 0.20
TnsC (1 to 280) TnsD (1 to 507) wt 0.014
  1. aDeletion analysis of TnsD and TnsC revealed that this protein-protein interaction activity is contained within TnsC 1 to 293 and TnsD 1 to 309.