Table 5 Yeast 2 hybrid assay shows that TnsC and TnsD can interact in vivo.
From: Characterization of the TnsD-attTn7 complex that promotes site-specific insertion of Tn7
Protein fragment | β-galactosidase activity | |
|---|---|---|
Fos | Jun | 5.0 |
E2F1 | RB | 1.0 |
Empty vector Gal4-AD | Empty vector Gal4-DB | 0.075 |
TnsC (1 to 555) wt | TnsD (1 to 507) wt | 1.0 |
TnsC (1 to 555) wt | TnsD (1 to 436) | 0.075 |
TnsC (1 to 555) wt | TnsD (1 to 399) | 4.5 |
TnsC (1 to 555) wt | TnsD (1 to 375) | 5.0 |
TnsC (1 to 555) wt | TnsD (1 to 350) | 4.5 |
TnsC (1 to 555) wt | TnsD (1 to 309)a | 4.5 |
TnsC (1 to 555) wt | TnsD (1 to 293) | 0.075 |
TnsC (1 to 555) wt | TnsD (8 to 507) | 0.075 |
TnsC (1 to 555) wt | TnsD (12 to 507) | 0.075 |
TnsC (1 to 555) wt | TnsD (16 to 507) | 0.075 |
TnsC (1 to 555) wt | TnsD (22 to 507) | 0.075 |
TnsC (1 to 555) wt | TnsD (1 to 507) wt | 1.0 |
TnsC (1 to 480) | TnsD (1 to 507) wt | 1.0 |
TnsC (1 to 384) | TnsD (1 to 507) wt | 1.0 |
TnsC (1 to 332) | TnsD (1 to 507) wt | 0.20 |
TnsC (1 to 293)a | TnsD (1 to 507) wt | 0.20 |
TnsC (1 to 280) | TnsD (1 to 507) wt | 0.014 |