From: Characterization of the TnsD-attTn7 complex that promotes site-specific insertion of Tn7
From dominant-negative TnsD screena | Relative transposition in presence of | Relative attTn7 binding in vivod | Relative attTn7 binding in vitro | |
---|---|---|---|---|
TnsD proteins | TnsABCb | TnsABCc | Â | Â |
No TnsD | 1.00 | 0 | < 0.00001 | - |
TnsD WT | 1.00 | 1.13 | 1.0 | 1.0 |
TnsD A18T | 0.05 | 0.01 | - | - |
TnsD P82L | 0.05 | 0.01 | - | - |
TnsD M96I | 0.10 | 0.30 | 0.005 | 0.3 |
TnsD G109E | 0.07 | < 0.001 | 0.0006 | 0.05 |
TnsD P147L | 0.05 | < 0.001 | 0.0016 | 0.07 |
TnsD R276C | 0.04 | < 0.001 | - | - |
TnsD H287Y | 0.06 | < 0.001 | - | - |
TnsD P417L | 0.08 | 0.04 | 0.0014 | 0.07 |
TnsD C447Y | 0.10 | 0.40 | 0.012 | 0.7 |
TnsD A486H | 0.06 | 0.04 | - | 0.1 |
TnsD A497I | 0.06 | 0.04 | 0.0005 | 0.05 |
Truncations | Â | Â | Â | Â |
TnsD Q227Z | 0.05 | < 0.001 | - | - |
TnsD Q305Z | 0.03 | < 0.001 | - | - |
TnsD Q306Z | 0.03 | < 0.001 | - | - |
TnsD Q340Z | 0.04 | < 0.001 | 0.0004 | < 0.05 |
TnsD W371Z | 0.08 | < 0.001 | - | < 0.05 |