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Table 2 Targeted mutations to the conserved CCCH residues in TnsD eliminates Tn7 transposition and TnsD-attTn7 binding.

From: Characterization of the TnsD-attTn7 complex that promotes site-specific insertion of Tn7

Protein

Relative attTn7 binding in vitro

Relative transposition frequency in vivo

TnsD wt

1.0

1.0a

TnsD C124S

0.05

< 0.001

TnsD C127S

0.05

< 0.001

TnsD C152S

0.05

< 0.001

TnsD C155S

0.05

< 0.001

  1. aThe transposition frequency in the presence of TnsD wt is 7.8 × 10-4.
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Mobile DNA

ISSN: 1759-8753