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Table 1 TnsD binding to attTn7 in vivo

From: Characterization of the TnsD-attTn7 complex that promotes site-specific insertion of Tn7

attTn7

position

Lysogeny frequencya

 

TnsD

induced

TnsD

uninduced

+23 → +58(wt)

7.4 × 10-5

5.4 × 10-7

+31 C→A

5.2 × 10-9

-

+42 T→G

3.6 × 10-8

-

+43 T→G

1.6 × 10-8

-

+51 T→G

5.2 × 10-9

-

  1. aChallenge assay phages carrying the indicated mutations in attTn7 were infected into cells expressing TnsD and the frequency of lysogeny measured; values are the average of three independent infections performed in parallel and relative to the wild type.
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Mobile DNA

ISSN: 1759-8753