Figure 1

IS911 genetic organisation transposition pathway. (A) IS911, donor DNA and target DNA are represented as bold, fine and dotted lines respectively. Small dark circles represent the two inverted repeats (IRs). Grey ellipses represent OrfAB: note that the stoichiometry is not shown. Arrows represent strand cleavage and transfer reactions that take place within the synaptic complex (SCA) or paired end complex (PEC). IS911 insertion is drawn as a nontargeted pathway [16, 31, 32]. In the synaptic complex B (SCB), proteins are represented as grey ellipses without implying OrfAB stoichiometry or the presence or absence of OrfA in the complex. (B) Inverted repeat left and right (IRL, IRR) sequences: orfA (translational phase 0) and orfB (phase -1). pIRL is indicated, and the frameshift site is shown as a dotted line. HTH motifs are designated as grey ovals and LZ motifs as an ellipse for each heptad. The M and DDE domains are also indicated. Domains present in both OrfA (100 aa) and OrfAB (382 aa) are indicated by light grey, and those in OrfAB alone by dark grey (see text). The two designed protein variant used in this study OrfAB1-149 (149 amino acids (aa)) and TrxA-OrfAB1-149 (308 aa) are represented. The dark box represents the TrxA tag. (c) Nucleotide sequence of the terminal IRL and IRR. Conserved nucleotides are shown on a grey background. The DNaseI footprint of OrfAB[1-149] is indicated schematically above and below the sequences[21].