Table 1 Two-hybrid plasmids
From: Two-hybrid analysis of Ty3 capsid subdomain interactions
Vector/plasmid | Nde I/Bam HI insert | Gag3 residues | Mutation(s) |
|---|---|---|---|
pGBK T7 (with Gal4-BD): | |||
pMZ2667 | Gag3 | 1-290 | WT |
pMZ2668 | Gag3 ΔSP | Δ207-233 | WT |
pMZ2669 | p27 | 1-233 | WT |
pMZ2670 | CA | 1-207 | WT |
pMZ2671 | CA-NTD | 1-135 | WT |
pMZ2672 | CA-CTD | 136-207 | WT |
pMZ2673 | NC | 234-290 | WT |
pMZ2676 | Gag3 | 1-290 | D60A/R63A (M4) |
pMZ2652 | Gag3 | 1-290 | E148A/K149A (M13) |
pMZ2688 | Gag3 | 1-290 | E190A/R191A (M18) |
pMZ2650 | Gag3 | 1-290 | G87A (MHR2) |
pMZ2651 | Gag3 | 1-290 | F93A (MHR4) |
pVB2832 | CA-CTD | 136-207 | E148A/K149A (M13) |
pVB2833 | CA-CTD | 136-207 | E190A/R191A (M18) |
pGAD T7 (with Gal4-AD): | |||
pMZ2677 | Gag3 | 1-290 | WT |
pMZ2678 | Gag3 ΔSP | Δ207-233 | WT |
pMZ2679 | p27 | 1-233 | WT |
pMZ2680 | CA | 1-207 | WT |
pMZ2681 | CA-NTD | 1-135 | WT |
pMZ2682 | CA-CTD | 136-207 | WT |
pMZ2683 | NC | 234-290 | WT |
pMZ2686 | Gag3 | 1-290 | D60A/R63A (M4) |
pMZ2655 | Gag3 | 1-290 | E148A/K149A (M13) |
pMZ2689 | Gag3 | 1-290 | E190A/R191A (M18) |
pMZ2653 | Gag3 | 1-290 | G87A (MHR2) |
pMZ2654 | Gag3 | 1-290 | F93A (MHR4) |
pVB2829 | CA-NTD | 1-135 | D60A/R63A (M4) |
pVB2830 | CA-NTD | 1-135 | G87A (MHR2) |
pVB2831 | CA-NTD | 1-135 | F93A (MHR4) |