Figure 2From: Creation of a novel telomere-cutting endonuclease based on the EN domain of telomere-specific non-long terminal repeat retrotransposon, TRAS1In vitro cleavage of human telomeric repeats by chimeric endonucleases. (a) Schematic representation of cleavage assay. pTR80, 80 TTAGGG repeats were inserted in the TA cloning site of pGEM-T Easy vector. pNTR, a non-telomeric sequence inserted in the same vector (see Methods). (b) Digestion patterns with various chimeric endonucleases. Plasmids (36 ng) were added with about 0.3 μM purified proteins, except for FN-T (about 0.02 μM), and incubated for 2 h at 25°C and separated by agarose gel electrophoresis. Lanes 1 ~ 15: pTR80/Sca I, lanes 16 ~ 30: pNTR/Sca I.Back to article page