Absence of eccDNA homologous to the dispersed Alu repeats. HEK-293 (human embryonic kidney cells) DNA, was cleaved with Eco RI followed by 'plasmid safe' digestion and analysed on a two-dimensional gel. Hybridization of the blot with Alu probe revealed only the arc that corresponds to linear DNA (A). Rehybridization of the same blot with Sst I probe revealed both linear DNA and eccDNA multimers (B). Arrowheads indicate the circular multimers.