Empty sites detected in Arabidopsis thaliana strains at positions occupied in Col by (a) Sadhu5-1 and (b) Sadhu5-1d1. Multiple sequence alignments of Col 5' and 3' sequences flanking the site of insertion along with sequences of strains that do not contain the insertion. Sequences corresponding to Sadhu element insertions have been removed. Genbank accession numbers for Sadhu5-1 sequences are EF535531 and EF535532. Genbank accession numbers for Sadhu5-1d1 sequences are EF535533, EF535534, EF535535, EF535536, EF535537, EF535538, EF535539, EF535540, EF535541, EF535542, EF535543, EF535544, EF535545, EF535546, EF535547, EF535548, and EF535549.