Schematic alignment of selected Sadhu subfamilies in strain Col. TSD sequences are different at different elements. Sizes of TSDs: TSD1, 11 base pairs (bp); TSD2, 12 bp; TSD3, 12 bp; TSD4, 10 bp; TSD5, 13 bp. Percentages correspond to sequence identity to the longest element in the subfamily. Sizes marked above each line represent positions relative to the gapped alignment and might be slightly different from the nucleotide length of element. (a) Sadhu5; (b) Sadhu6; (c) Sadhu2; (d) Sadhu3; (e) Sadhu8-1 versus Sadhu8L3. TSD = target site duplication.