A proposed mechanism for IS607-family serine transposases
© Boocock and Rice; licensee BioMed Central Ltd. 2013
Received: 22 June 2013
Accepted: 7 October 2013
Published: 6 November 2013
The transposases encoded by the IS607 family of mobile elements are unusual serine recombinases with an inverted domain order and minimal specificity for target DNA.
Structural genomics groups have determined three crystal structures of the catalytic domains of IS607 family transposases. The dimers formed by these catalytic domains are very different from those seen for other serine recombinases and include interactions that usually only occur upon formation of a synaptic tetramer.
Based on these structures, we propose a model for how IS607-family transposases could form a synaptic tetramer. The model suggests that, unlike other serine recombinases, these enzymes carry out sequence-specific DNA binding and catalysis in trans: the DNA binding and catalytic domains of each subunit are proposed to interact with different DNA duplexes. The model also suggests an explanation for the minimal target DNA specificity.
KeywordsIS607 Serine recombinase Site-specific recombinase Structure Transposase Transposition mechanisms
The serine transposases encoded by IS607-type insertion sequences represent a poorly understood branch of the serine recombinase family. Serine recombinases all share a common catalytic domain that includes the eponymous serine that is the active site nucleophile. The most intensively studied branch of this family, and the only one for which extensive structural information is available, comprises the “canonical” resolvase/invertase group ; these catalyse resolution of transposition cointegrates and replicon dimers, or inversion of DNA segments. A second branch that has also been biochemically characterized comprises the large serine recombinases, which include a number of bacteriophage integrases and some transposases. For both of the characterized groups, the catalytic domain is always found at the N-terminus of the protein and is followed by a sequence-specific DNA binding domain: a simple helix-turn-helix for the resolvase/invertase group, or a much larger bipartite domain in the large serine recombinases (hence their name) . However, the IS607-family serine transposases carry a predicted helix-turn-helix DNA binding domain at the N-terminus, with the catalytic domain at the C-terminus (Figure 1c) [2, 5, 10].
For the well-characterized serine recombinases, activation entails a pair of inactive cutting dimers undergoing large conformational changes as they come together to form a catalytically active tetramer. In the resolvase and invertase systems, activation is triggered by formation of a large synaptic complex that has a defined topology and includes additional copies of the recombinase and/or other DNA bending proteins [20, 21]. This requirement can be bypassed by mutations that tip the conformational balance from the inactive dimer normally favoured by the isolated wild type (WT) protein to the catalytically active tetramer [10, 22, 23]. Integrases of the large serine recombinase family can form synaptic complexes without accessory factors, but usually only if they involve certain pairs of DNA sites (e.g., attP, and attB, the attachment sites found in the phage and bacterial DNA, respectively) .
It has been unclear how to apply the lessons learned from the well-characterized serine recombinases to the IS607-family serine transposases. For instance, in the canonical serine recombinases, the catalytic and DNA binding domains of each protomer interact with the same DNA half-site [26, 31], but this is difficult to model for enzymes such as IS607 transposase where the DNA binding domain is N-terminal to the catalytic one, and attached by a rather short linker (~6aa for ISC1904). This geometry places the DNA binding domain (DBD) on the opposite side of the catalytic domain from the active site. Additionally, the current paradigm in which each of the four subunits binds one copy of a specific sequence motif (a “half site”) is hard to reconcile with the lack of target specificity shown by IS607-family transposases. Recently, structures of the catalytic domains of three different archaeal IS607-family serine transposases, including that from ISC1904 , have been determined by the Midwest Center for Structural Genomics (PDBids 3ilx, 3lhk and 3lhf). These reveal a very different dimer architecture than previously observed for other serine recombinases and suggest a different pathway for formation of an active tetramer.
The IS607-family dimer is a “rotating” rather than a “cutting” pair
If the resolvase tetramer were to be split into two rotating dimers, a large hydrophobic surface would be exposed (Figure 3). In the serine transposase dimer, the equivalent surface is covered by the C-terminal portions of the E helices that fold back against it (Figure 4a). These observations suggested a model for the transposase in which a full tetramer is assembled on a single DNA crossover site. As described below, such a tetramer can be assembled by maintaining the packing between E helices within each dimer and by rotating about two flexible points within each subunit.
Modelling an active IS607-family transposase tetramer
We anticipate that all IS607-family elements use a 'standard’ serine recombinase strand exchange mechanism (Figure 2; ) and transpose via a circular intermediate, similar to the circular forms of bacteriophages that use a large serine recombinase for integration/excision. In the circular form, the two ends of the mobile element would be joined to form a new crossover site through a specific 'overlap’ dinucleotide ('GG’ for IS607 ). Evidence for a circular form of IS607 was obtained by PCR in an E. coli-based transposition assay (NDF Grindley, personal communication). Recombination between the crossover site in the circular intermediate and a matching dinucleotide in the target DNA would insert the element into a new genomic location. Here, we propose a pathway for integration that can easily be extrapolated to the excision step.
To find a good model for the DBD, which was not included in any of the deposited serine transposase structures, we used the PROF routine of PredictProtein to predict its secondary structure . This was consistent with a winged helix-turn-helix, with two short helices followed by a β-hairpin wing and a third short helix. The SoxR repressor begins with just such a DNA-binding motif and is also the top hit found by the Phyre2 threading server [35, 36]. We therefore used a truncated version of the SoxR structure, with the DNA it was co-crystallized with, to model the DBD of the full-length ISC1904 transposase-DNA complex. The third helix of the transposase’s DBD is predicted to end at residue 47; this implies that there is a 6-residue linker before the catalytic domain, which becomes ordered in the crystal at residue 54.
Straightening the E helices of the dimer exposes a hydrophobic surface, which we propose interacts with that of a similar dimer (Figure 5b, third panel), triggering tetramer assembly. The order of the conformational changes proposed in Figure 5 is arbitrary. However, it is plausible that sequence-specific major groove binding by one subunit’s DBD would cause the C-terminal part of its partner’s E-helix to flip into the adjacent minor groove. Synergistic binding of a second dimer would result in a full tetramer bound to one DNA segment. Tetramer formation would force the other pair of E-helices (green in Figure 5b) into the extended conformation, ready to bind target DNA. Since the second set of DNA-binding moieties is thus pre-assembled, the tetramer’s affinity for target DNA of nearly-random sequence would be greatly increased over that of a single inactive transposase dimer. Note that all DNA binding proteins have some affinity for random-sequence DNA, although it can be orders of magnitude weaker than that for specific sequences. Our model implies that the serine transposase’s affinity for specific vs. non-specific DNA is tuned such that the affinity of a single DBD for non-specific DNA is too weak to be physiologically significant, but, due to cooperativity, a pre-assembled array of two DBDs plus two E helices binds non-specific target DNA tightly enough to be functionally relevant.
Figure 6 shows a ribbon drawing of the final model and a superposition of it onto the γδ resolvase tetramer structure. The catalytic domains and E-helices overlap quite well, with the only major difference lying in the placement of the DBDs. Note that the E-helix interactions of the initial dimer were maintained throughout the modelling. There is precedent for the type, if not the scale, of the inter-domain motions needed to construct the model. In several other structures, the E-helix bends and/or becomes disordered at the position where it folds back on itself in the IS607-family dimers [24–26] and PDBid 3 g13. Rotation of the catalytic domain core relative to the E-helix occurs in the transition from inactive dimer to active tetramer for both γδ and Sin resolvases and triggers assembly of the active site [19, 28, 29].
In the serine transposase case, an extra level of regulation may keep the dimer inactive until the proper complex is assembled: the active sites within the catalytic domain cores are physically occluded by the E-helices. The inhibitory interaction between the cores and E-helices is stabilized by two negatively charged side chains that interact with the conserved arginines of the active site (Figure 4a). This pair of negatively charged residues is highly conserved within the serine transposases but not within the larger serine recombinase family.
Another question is whether or not a tetramer assembled on one crossover site would repeatedly cleave and religate that site even in the absence of target DNA. Kinetic experiments suggested that Sin resolvase tetramers were catalytically active even when only bound to one DNA segment. However, those experiments bypassed the natural assembly pathway for Sin . The serine transposases may have evolved an additional regulatory mechanism to avoid making double strand breaks until both DNA partners are present, which would be an interesting question to address experimentally. Preassembly of an active complex that captures a target site (of varying specificity) has good precedent in otherwise unrelated recombination systems, e.g., phage lambda integrase and the DDE family of transposases and retroviral integrases [39, 40].
Our modelling exercise demonstrates that while the assembly pathway may be very different, the final activated tetramer formed by IS607-family serine transposases may be very similar to that formed by canonical serine recombinases. However, it would differ in that each subunit would act in trans – that is, the catalytic and DNA binding domains would interact with different DNA duplexes.
Modelling was carried out by manipulating the relevant structures manually in Pymol (The PyMOL Molecular Graphics System, Version 1.3 Schrödinger, LLC).
DNA binding domain
Protein database identification
This work was funded by a Functional Supplement to NIH R01 GM086826 (to PAR) and by the Wellcome Trust (MRB). We thank S-J Rowland and James Fuller for comments on the manuscript, and NDF Grindley for communicating unpublished data.
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