Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalis
© Yergeau et al; licensee BioMed Central Ltd. 2011
Received: 22 July 2011
Accepted: 24 November 2011
Published: 24 November 2011
The Sleeping Beauty (SB) transposon system has been used for germline transgenesis of the diploid frog, Xenopus tropicalis. Injecting one-cell embryos with plasmid DNA harboring an SB transposon substrate together with mRNA encoding the SB transposase enzyme resulted in non-canonical integration of small-order concatemers of the transposon. Here, we demonstrate that SB transposons stably integrated into the frog genome are effective substrates for remobilization.
Transgenic frogs that express the SB 10 transposase were bred with SB transposon-harboring animals to yield double-transgenic 'hopper' frogs. Remobilization events were observed in the progeny of the hopper frogs and were verified by Southern blot analysis and cloning of the novel integrations sites. Unlike the co-injection method used to generate founder lines, transgenic remobilization resulted in canonical transposition of the SB transposons. The remobilized SB transposons frequently integrated near the site of the donor locus; approximately 80% re-integrated with 3 Mb of the donor locus, a phenomenon known as 'local hopping'.
In this study, we demonstrate that SB transposons integrated into the X. tropicalis genome are effective substrates for excision and re-integration, and that the remobilized transposons are transmitted through the germline. This is an important step in the development of large-scale transposon-mediated gene- and enhancer-trap strategies in this highly tractable developmental model system.
Amphibian model systems have provided a wealth of information on the molecular mechanisms controlling early vertebrate development. Frogs of the Xenopus genus are particularly well suited for embryological study as these animals adapt well to captivity and the females can be induced to lay large numbers of eggs throughout the year. The most commonly used amphibian model is the South African clawed frog, X. laevis. Genetic manipulation of this species is not practical due to the long generation time (> 1 year) and the pseudo-tetraploid nature of the genome. Another species of the Xenopus genus, X. tropicalis, shares the embryological advantages of its South African cousin and is better suited for genetic studies as it is a true diploid and has a relatively short generation time (approximately 6 months). The potential of applying modern genetics to this classical embryological model system has resulted in the rapid development of genomic tools for X. tropicalis in recent years (reviewed in [1, 2]), and the publication of the genome sequence .
Our studies have focused on using the class II DNA 'cut-and-paste' transposable elements to modify the frog genome for gene- and enhancer-trapping and for insertional mutagenesis [4–9]. Transposable elements have been used for many years to experimentally modify the genomes of plants and invertebrates and, more recently, have been applied to vertebrate model systems [10, 11]. Transgenesis with non-autonomous transposable elements offers advantages over other transgenic methodologies. First, transposable elements efficiently integrate into the target genomes. Second, as the transposon is excised from the donor plasmid prior to integration, plasmid sequences, which may cause epigenetic silencing [12, 13], are not integrated at the targeted locus. Third, once integrated into the genome, the transposon transgene is an effective substrate for excision and re-integration (remobilization) following re-expression of the cognate transposase enzyme. The ability to remobilize transposons resident in the genome can be used for a variety of applications, including large-scale transposon 'hopping' screens using gene- or enhancer-trap constructs.
Remobilization of a non-autonomous transposon transgene is achieved by expressing the transposase enzyme in the same cell harboring the transposon. This can be achieved by simply injecting fertilized one-cell embryos from the outcross of transposon transgenic animals with mRNA encoding the transposase. As development proceeds, the injected mRNA is translated by the host cell and catalyzes the excision and re-integration reactions. This approach has been used successfully with the Tol2 transposon system in fish and frogs [7, 14–16]. Another approach is to develop transgenic animals that express the transposase enzyme under the control of tissue specific promoters and to cross these animals with those that harbor a transposon substrate to generate double-transgenic progeny. This approach has been used very successfully for somatic remobilization of the Sleeping Beauty (SB) transposon to identify cancer genes in mice [17, 18]. Outcross of the transposase enzyme and transposon substrate double transgenic animals can result in novel remobilization events in the progeny [19–23].
We, and others, have used a co-injection strategy with the SB transposon system to generate transgenic Xenopus that express fluorescent proteins under the control of ubiquitous or tissue-specific promoters [4, 6, 25]. The integration events generated by this method in the frog are not caused by the simple transposition of the transposon from the plasmid into the frog genomic DNA. Analysis of the integration sites indicated that several copies of the transposon, and parts of the flanking plasmid sequence, are introduced at discrete loci as small-order concatemers. This unexpected non-canonical integration mechanism makes cloning the integration site complicated and time consuming . Although the integration events generated by the co-injection strategy resulted in non-canonical integration, we next investigated whether SB transposons stably integrated into the X. tropicalis genome are effective substrates for remobilization. Using a double-transgenic strategy, we show that SB transposons in the frog genome can be remobilized following re-expression of the SB transposase and that the remobilized integration events occur via canonical transposition.
Generation and analysis of transgenic X. tropicalis expressing SB 10 transposase
Generation of double-transgenic 'hopper' frogs
The pT2 βGFP 8F founder harbors two independently-segregating alleles: a concatemer of three SB transposons integrated at a single locus on scaffold 57, at base number 2456981 (57:2456981) of the JGI X. tropicalis genomic sequence v4.1 assembly, and another allele with a single-copy transposon integration . Thus, the F2 hopper frogs inherited either one, or both, of the 8F integration events. Southern blot analysis of progeny from 8Fhopper♂35 indicated that this double-transgenic hopper had inherited the trimeric concatemer of pT2 βGFP on scaffold 57 alone. Double-transgenic (RFP+/GFP+) progeny (F3) from the outcross of 8Fhopper♂35 were raised and outcrossed, and the resulting progeny (F4) were analyzed for modification of the parental pT2 βGFP locus (Figure 2).
Genomic DNA harvested from GFP-positive progeny from double transgenic (pT2 βGFP8F:CAGGS-SB 10;γcRFP) 8F hopper frogs was analyzed by Southern blot. Digestion of genomic DNA from pT2 βGFP 8F tadpoles with Bgl II resulted in three bands when the blot was hybridized with a GFP probe (Figure 4c). Changes in the Southern blot hybridization pattern were used to determine whether the parental concatemer had been altered by expression of the SB transposase. Analysis of progeny from the outcross of F3 double transgenic hopper frogs with wild-type animals indicated that most of the progeny had inherited the unaltered pT2 βGFP 8F parental concatemer (Figure 4c; lanes 3, 4 and 5). Examples of germline remobilization of the pT2 βGFP transposon from 'GFP-bright' tadpoles (Figure 4a; tadpoles 1 and 2) harvested from the outcross of 8Fhopper♂58 are shown in Figure 4c (lanes 1 and 2, dashed arrows). This data indicates that, as predicted, the GFP-bright individuals in the outcross population of the hopper frogs represent tadpoles that have modified the parental transposon donor locus. Thus, remobilized animals can be identified in the outcross population by simply observing the tadpoles for changes in GFP intensity. Outcross of eleven 8F hopper double transgenic frogs indicated that the frequency of remobilized progeny varied from 0.07% to 0.71% (Figure 4b). The variation in the remobilization activity between individual hopper frogs likely reflects subtle differences in epigenetic modification of the substrate and enzyme transgenes in each animal that may alter the activity of the excision and reintegration reactions.
Integration site analysis for remobilized progeny from 8F hoppers.
Right indirect repeat/direct repeats flanking sequence
5' flanking gene
3' flanking gene
Pre-sorting tadpoles based on GFP intensity may underestimate the total remobilization activity if the re-integration event resulted in GFP expression that was not markedly different from the parental expression. To test this, we outcrossed a double transgenic hopper frog (8Fhopper♂51) and analyzed all of the GFP-positive progeny by Southern blot. The 8Fhopper♂51 frog inherited both of the pT2 βGFP transposon alleles from the 8F founder. The progeny from this 8Fhopper♂51 outcross displayed GFP expression patterns and intensities that were indistinguishable from that of the parental alleles (data not shown). From the 677 GFP-positive progeny analyzed by Southern blot, we identified four excision-only events and two remobilizations. Samples of genomic DNA where changes were evident by Southern blot analysis were used in EPTS LM-PCR to clone the integration site of the remobilization events. In this experiment, the remobilization frequency was 0.3% (two remobilization events out of 677 GFP-positive tadpoles). These data indicated that the actual remobilization frequency may be somewhat higher than that estimated by simple visual inspection of the GFP-positive progeny. The observed rate of excision-only events in this outcross population was 4 out of 677, that is, 0.6%.
Scoring the outcross progeny of hopper frogs for changes in GFP intensity may also overestimate the remobilization frequency, as this method may not distinguish between remobilization events and excision-only events. We analyzed 25 GFP-bright tadpoles from the outcross of 8F and 7M (see below) hopper frogs, by Southern blot analysis and by cloning the novel insertion sites by EPTS LM-PCR. Only one GFP-bright tadpole had an excision-only modification of the parental transposon donor locus (4%); 24 GFP-bright tadpoles (96%) had re-integration events that were evident by novel bands on the Southern blot and by cloning the sequences flanking the canonical re-transposition events. Thus, while it is possible to identify excision-only events by changes in GFP expression, the vast majority of GFP-bright progeny represent re-integration events.
Remobilization of transposons resident in the genome may result in chromosomal rearrangements near the donor locus [34–41]. In mice, germline remobilization of SB transposons from a high-copy number (approximately 30 copies) concatemer resulted in frequent alteration of the genomic sequences flanking the transposon donor locus; nine out of nine remobilized pedigrees examined displayed genomic alterations spanning 105 bp to 107 bp flanking the donor site . To determine whether SB remobilization in the frog resulted in similar genomic alterations near the donor locus, we examined the sequences flanking the 8F donor locus by PCR. Genomic DNA samples from eight remobilization events and eight excision-only events were used to amplify the sequences flanking the 5' and 3' ends of the 8F concatemer on chromosome 6. In each case, genomic PCR using primers that amplify the 5' and 3' junctions of the 8F locus generated the appropriate sized products (data not shown), indicating that the sequences directly flanking the donor locus are intact following excision of pT2 βGFP transposons from the donor site.
Sequence analysis of the re-integration target sites indicated a similar base distribution flanking the canonical TA dinucleotide to that observed with SB integration in mammalian genomes [42, 43] (Figure 5d). Transposons of the Tc1/mariner family, including SB, integrate at TA dinucleotides. The consensus sequence for SB integration in frogs, as in mammals, is a palindromic ATATA TAT sequence, where the canonical TA target is in bold, although none of the re-integration events observed in the frog have this exact palindrome.
GFP-bright progeny from the outcross of 8F hopper frogs were raised to the adult stage, and outcrossed to demonstrate that the remobilized transposon alleles are stably transmitted through the germline. Genomic DNA was harvested from GFP-positive and GFP-negative siblings and used for Southern blot analysis and for cloning the novel integration site by EPTS LM-PCR. For example, remobilized female frog 62E3 produced GFP-bright progeny and integration site analysis showed a single copy of the pT2 βGFP transposon on scaffold 140 (140:1237072). The novel re-integration event was on the same chromosome as the donor locus (chromosome 6, linkage group 2), approximately 1 cM from the parental 8F concatemer, and represents a local hop (data not shown). The 62E3 integration event was in the 3' UTR of a muscle-related coiled coil protein (GenBank accession number XM_002935280.1) gene.
Integration site analysis for remobilized progeny from 7M Hoppers
Right IR/DR flanking sequence
5' flanking gene
3' flanking gene
To date, four 7M female hopper frogs have been outcrossed, and the mean remobilization rate from these animals is 2.54%. This may reflect individual differences in excision and reintegration activities between different hopper animals, or it may indicate that remobilization, driven by the CAGGS-SB 10 transgene, is more efficient in the female germline (mean 2.54%; n = 4) than in the male germline (mean 0.76%; n = 9, unpaired Student's t-test, P = 0.0088, degrees of freedom, 11). With the 8F hoppers, we observed a modest increase in the mean remobilization efficiency with the female hoppers (0.56%; n = 2) compared to the male hoppers (0.25%; n = 7); however, due to the small sample size, this may not be statistically significant (Student's t-test P = 0.25, degrees of freedom, 1).
The 7M pT2 βGFP concatemer is located on scaffold 38 that maps to chromosome 10 (Figure 7b and 7c). Genomic DNA harvested from representative GFP-bright tadpoles from the 7M hopper outcrosses was analyzed by Southern blot and the novel integration sites were cloned by EPTS LM-PCR. As noted for the remobilized 8F hopper progeny above, the novel integration events from the 7M hoppers were canonical SB-mediated transposition events. As determined for the remobilization events from the 8F hopper frogs, a strong bias for local re-integration was observed for the 7M hoppers; re-integration events on the same scaffold (scaffold 38) as the transposon donor were cloned from the GFP-bright tadpoles.
SB transposons can be remobilized in X. tropicalis
Here, we demonstrate that SB transposons integrated into the frog genome are effective substrates for remobilization following re-expression of the SB transposase. Unlike the integration events observed in the co-injection strategy that were mediated by a complex non-canonical mechanism, the remobilized SB transposons re-integrated via canonical SB-mediated transposition. The observed frequency of excision and subsequent re-integration of the parental pT2 βGFP transposon was low (on average, less than 1%). Our data in X. tropicalis is similar to that observed in other in vitro and in vivo[19, 46, 47] systems, where low-copy number transposon donor sites served poorly as substrates for remobilization. In mammals, increasing the number of transposon substrates by using donor sites that contain high-order concatemers resulted in increased remobilization activity [19, 46]. For example, in AB1 embryonic stem cells, a single SB transposon was 'knocked in' to the Hprt gene on the mouse X chromosome and subsequent transient expression of SB transposase (SB 10) resulted in a transposition rate of circa 3.5 × 10-5 events per cell per generation . In mice, low-copy number SB transposon donor sites result in a low frequency of remobilization events that are passed through the germline [19, 47]. For example, single-copy SB transposon donors result in novel re-integration sites in approximately one embryo in every one hundred (around 1%) in an outcross of double transgenic 'seed' mice . Increasing the number of transposon substrates by using donor sites that contain high-order concatemers resulted in increased remobilization activity; however, Geurts and colleagues noted that there was not a linear correlation between donor site copy number and remobilization activity . This suggests that other factors, such as the methylation status, and other local chromatin-environment factors, may also influence the ability of integrated SB transposons to serve as substrates for remobilization. Horie and colleagues observed that low-copy number SB transposon concatemers served very poorly as substrates for remobilization in mice, and that the presence of more copies of the SB transposon in the concatemer acted synergistically to increase the frequency of excision and re-integration. With a donor site that contained around 20 copies of the transposon substrate, a remobilization rate of 1.25 transpositions per genome per animal (125%) was observed . Keng and colleagues also reported a similar transposition frequency when using double transgenic mice that contained either 20 copies (1.16 transpositions per GFP-positive mouse) or 100 copies (1.14 transpositions per GFP-positive mouse) of the substrate transposon in the donor concatemer .
In this study, we used low-copy number donor sites as substrates for remobilization as the integration events observed with the plasmid-mRNA co-injection strategy were mediated by a complex, non-canonical integration mechanism . We reasoned that, if a similar non-canonical mechanism were used in the remobilization step, starting with a simple substrate would help facilitate cloning of the remobilization event. Although the remobilization frequency observed in the outcross of the double transgenic frogs is low, the re-integration events are canonical SB-mediated transpositions. There are several strategies available to increase the frequency of remobilization events in the frog genome. Increasing the copy number of SB transposon substrates will likely significantly increase the frequency of novel re-integration events in the outcross progeny from hopper frogs. Transgenic frogs with multiple copies of the pT2 βGFP transposon have been generated (7M and ♀622E) that each harbor more than seven copies of the pT2 βGFP transposon . The total number of transposon substrates in each hopper line can be further increased by incrossing the hopper lines with other pT2 βGFP founders that contain multiple copies of the SB transposon. In addition to donor site transposon copy number, the transgenic transposase enzyme may also influence the remobilization activity in the frog. The transgenic SB enzyme frog described here was generated using the first generation SB transposase (SB 10; ). In recent years, several hyperactive mutant forms of the SB enzyme have been developed, including SB 11 that has approximately three-fold higher activity  and SB 100X that has a 100-fold increase in enzymatic activity when compared to SB 10 . In addition to the choice of modified enzyme, different promoters with varying transcriptional activity could be used to drive expression of the SB transgene to enhance the rate of germline remobilization. This may not be as simple as finding the most powerful promoter and/or enhancer available, as SB is sensitive to overproduction inhibition, where increasing levels of enzyme impair the overall transposition efficiency .
Why are different integration mechanisms observed with the co-injection and the double transgenic strategies?
There are several possible reasons for the different integration mechanisms used in the two SB-mediated methods, that is, injection-mediated and breeding-mediated transposition. First, the concentration of the substrate is vastly higher in the injection method where approximately 75 pg (around 10.8 × 106 copies) of the plasmid harboring the SB transposon substrate was co-injected with SB mRNA. By comparison, the pT2 βGFP 8F founder used in the transgenic remobilization strategy contained three copies of the SB transposon. The SB transposase catalyzes transposition as a dimer of dimers (tetramer) bound to the indirect IR/DR elements that flank the transposon . The massive excess of substrate present in the injection-mediated strategy may prohibit the correct assembly of transposase on the substrate and may result in non-canonical enzymatic activity. Also, the integrated transposon may also be a better substrate for SB activity due to DNA methylation and heterochromatinization [50, 51]. Recent studies have shown that CpG methylation and supercoiling of SB transposon-harboring plasmids result in highly efficient transposition by the co-injection method in mammals when compared to non-methylated linear plasmid DNA donors . Finally, the differences in the integration mechanisms observed with the two strategies may reflect differences in the availability of host factors for SB transposition in the developing gametes and the early-cleavage stage Xenopus embryos.
Potential uses for SB remobilization in X. tropicalis
The demonstration that SB transposons stably integrated into the frog genome are effective substrates for remobilization is an important step in the development of large-scale insertional mutagenesis and enhancer- or gene-trap screens in the frog. The breeding-based remobilization strategy described here provides a simple and robust method for generating novel transgenic lines without the need for labor- and skill-intensive micro-injection methodologies. The frog provides several important advantages for transposon-based genetic screens. First, each outcross can generate several thousand progeny. The high fecundity of X. tropicalis indicates that, even if the remobilization frequency is low, multiple novel re-integration events can be identified in a single outcross.
A second advantage is that Xenopus have a long lifespan in captivity that may reach two decades or more, and the animals remain fertile for more than ten years. This has important implications for remobilization strategies, as double transgenic hopper frogs can be maintained and outcrossed at regular intervals for many years; male frogs can be outcrossed every two weeks and females every two months. Laboratories with limited animal holding space can, with a small cadre of hopper frogs, perform large-scale enhancer- or gene-trap screens, keeping only those tadpoles with interesting GFP expression profiles. The long lifespan of the hopper frogs may also have important implications if epigenetic silencing of the transgenic transposase locus is identified over a series of generations. Stably integrated transgenes are frequently subjected to epigenetic silencing over successive generations [12, 53, 54]. Silencing of the transposase locus would likely result in abolishment of the hopping activity. As each generation of frog lives for many years, having to regenerate new lines for hopping strategies is not likely to be a problem in this species.
The propensity of SB to catalyze local hopping events is a third advantage that can be exploited to generate insertional mutants of genes near the donor locus. In mice, approximately 75% of remobilized SB transposons re-integrate within 3 Mb of the donor locus . Our data with remobilization of the 8F locus indicates that local hopping is also a feature of the SB transposition in X. tropicalis. Transgenic SB transposon frogs that have integrations in gene-dense regions of the genome can be used as donors for insertional mutagenesis strategies, as re-integration within a nearby gene may disrupt the normal activity of that locus. In the example presented here, the 8F transposon donor is located on scaffold 57, which maps to X. tropicalis chromosome 6 and is syntenic with human chromosome 7 (Figure 6a). It is in a gene dense region with approximately 50 genes in the 3 Mb flanking the transposon donor. The genome size of X. tropicalis is approximately one-half that of the human genome , while the gene content of the frog is similar to that of man. Thus, the overall gene density is relatively high in the frog. Different DNA 'cut and paste' transposon systems offer unique advantages for manipulating the vertebrate genome. For example, the local hopping activity of SB can be exploited to saturate the genomic sequences flanking the transposon donor locus with novel re-integration events. We have recently demonstrated that Tol2 transposons stably integrated into the frog genome are effective substrates for remobilization . The local hopping activity of Tol2 is less pronounced than that of SB; approximately 20% of Tol2 re-integration events occur near the donor locus, compared to approximately 80% for SB. Using nested transposon substrates, with, for example, an SB transposon cloned within a Tol2 element, genome-wide remobilization screens could be performed using Tol2 to randomly distribute the dual substrate throughout the genome, with subsequent SB remobilization to locally saturate regions of interest with novel insertion events. In the study described here, we have used a simple ubiquitous promoter element to drive expression of the GFP reporter. Substrate transposons that harbor potentially more mutagenic elements, such as polyadenylation trap elements , can be used to efficiently disrupt the activity of the 'trapped' gene.
Finally, the frog is an excellent model for embryological and biochemical studies due to its small size, simple husbandry and the ease of manipulating embryos at all stages of development. Furthermore, as a tetrapod species, X. tropicalis shares a similar body plan with mammals, allowing analysis of developmental processes that are unique to higher vertebrates, such as limb and digit pattern formation. Combining these features with a simple and robust method for generating novel transgenic lines will provide valuable tools to apply to this highly tractable developmental model system.
SB transposons stably integrated into the X. tropicalis genome are substrates for remobilization
Co-injection of plasmid DNA harboring a SB transposon together with mRNA encoding the SB transposase results in efficient transgenesis of X. tropicalis. The integration events mediated by this co-injection approach are complex and frequently contain low-order concatemers of the transposon. In this study, we demonstrate that SB transposons stably integrated in the frog genome are effective substrates for remobilization. Transgenic frogs that express SB 10 transposase in the germline were bred with SB transposon frogs and the double transgenic progeny were outcrossed to wild-type animals. Remobilization events were readily identified by increased GFP expression in the offspring where the parental transposon concatemer had been modified by the SB 10 enzyme. Integration site analysis of the GFP-bright progeny indicated that the transposon re-integration events had occurred via a canonical cut and paste mechanism. The rate of remobilization observed in the frog was similar to that observed in other species when a low copy number concatemer was used as the transposon donor.
SB transposon remobilization as a tool for genetic manipulation of X. tropicalis
The diploid frog X. tropicalis offers several advantages for large-scale forward genetic screens in a tetrapod model, including vast numbers of progeny per spawn, long lifespan and availability of genomic resources including the genome sequence and genetic map. Here, we have demonstrated that we can exploit the cut and paste activity of SB transposase to generate novel transposon transgenics by simply breeding the double-transgenic hopper frogs. Novel transposon lines are readily identified by changes in GFP reporter expression in the remobilized progeny compared to the parental GFP pattern. The local hopping activity of SB can be exploited to saturate genomic regions that flank the transposon donor sites. The ability to generate thousands of progeny in each outcross, combined with the ease of identifying novel insertion events, will allow large-scale SB-mediated screens to be performed in X. tropicalis.
Plasmids and generation of transgenic lines
The generation of the pT2 βGFP construct and the transgenic pT2 βGFP X. tropicalis line 8F have been described previously . The pCAGGS-SB 10 construct was a gift from Dr David Largaespada . A 3,613 bp Hin cII/Bam HI fragment containing the promoter/enhancer and the SB 10 transposase from pCAGGS-SB 10 was cloned in pBluescript SK+ (pBS-SK+) to generate pBS-CAGGS-SB 10. The 2.2 kb X. laevis γ1 crystallin promoter driving dsRed construct was a gift from Dr Robert Grainger (2.2 γ1 crystallin-RFP; ). An approximately 3.5 kb γ1-crystallin promoter-RFP fragment was PCR amplified from 2.2 γ1 crystallin-RFP using primers DSR1 5'-GTAAGCGGCAGGGTCGGA-3' and DSR2 5'-GCCTCGAGCGATTTCGGCCTATTGGT-3', cloned into pGEM-Teasy (Promega, Madison, WI, USA), and fully sequenced, to yield pGEM-γcRFP. An approximately 3.5 kb Sac I restriction fragment from pGEM-γcRFP encoding the γ1 crystallin-RFP reporter was cloned into the unique SacI restriction site of pBS-CAGGS-SB 10. A single clone was selected with the two mini-genes oriented tail-to-tail in the pBS-SK plasmid (pCAGGS-SB 10;γcRFP; Figure 1a). The pCAGGS-SB 10;γcRFP construct was linearized with Sca I and injected in vitro into X. tropicalis fertilized embryos at the one-cell stage (500 pg of linear plasmid DNA in 3 nL of water) as described previously [6, 8]. Tadpoles were scored for expression of RFP in the lens after stage 40 . RFP-positive tadpoles (27 positive from 570 injected, 4.7%) were selected and raised to adulthood and outcrossed to determine germline transmission of the transgene. Male frog CAGGS-SB 10;γcRFP 2M produced progeny that expressed RFP robustly in the lens and was selected for further analysis.
Husbandry and micro-injection of X. tropicalis
X. tropicalis tadpoles were maintained at 28°C in static tanks and were staged according to Nieuwkoop and Faber . Adult animals were housed in a recirculating aquarium at 26°C. Transgenic adult frogs were identified by implanting a radio-frequency identification microchip (microSensys GmbH, Erfurt, Germany) beneath the skin of the dorsal surface of each animal . The unique 16-digit alphanumeric sequence encoded on each chip provides a convenient method for identifying individual animals throughout their lifespan. Female X. tropicalis animals were pre-primed with a 1:5 dilution of human chorionic gonadotropin (hCG) overnight, and primed the day of injection with 200 U of hCG. Fertilized eggs were obtained by natural matings. Injected eggs were allowed to heal at 28°C and transferred to tanks for growth at 28°C . This project was approved by St. Jude Children's Research Hospital's Institutional Animal Care and Use Committee.
RT-PCR analysis of SB expression
Total cellular RNA was isolated using the RNAeasy kit (Clontech, Mountain View, CA, USA) from individual RFP-positive and RFP-negative stage 40 embryos generated by outcross of CAGGS-SB 10;γcRFP 2M. First strand cDNA was synthesized and used as template for 32P-labelled PCR reactions as described previously . Primers for SB 10 amplification (SB3 5'-GCCGCTCAGCAAGGAAGA-3' and SB4 5'-GAAGACCCATTTGCGACCAAG-3') annealed at 56°C and produced a 383 bp fragment. Primers to Xenopus α-actin were used as a control for RNA recovery.
Western blot analysis of SB 10 protein in tissues harvested from transgenic frogs
Whole embryo or adult tissue samples were snap frozen in a dry ice and ethanol bath and stored at -80°C. 100 μl of RIPA buffer (150 mM sodium chloride, 50 mM tris(hydroxymethyl)aminomethane with hydrogen chloride pH 7.5, 1% (v/v) nonyl phenoxypolyethoxylethanol (IPEGAL), 0.5% (w/v) sodium deoxycholate, 0.1% (w/v) sodium dodecyl sulfate, 1X Complete Protease Inhibitor Cocktail (Roche, Indianapolis, IN, USA)) was added to the frozen samples, mixed by vortex, extracted with Freon (200 μL of 1,1,2-trichlorotrifluroethane (Sigma-Aldrich, St. Louis, MO, USA)) and centrifuged at 16,100 × g for 15 minutes at 4°C. The upper phase was transferred to a fresh tube and the protein concentration was measured using the Bradford assay (Bio-Rad, Hercules, CA, USA). Aliquots of each sample were diluted with an equal volume of 2× Laemmli Sample Buffer containing β-mercaptoethanol (Bio-Rad) and denatured by heating at 100°C for 5 minutes. Proteins were separated by electrophoresis on 4% to 15% (w/v) Criterion precast polyacrylamide gels (Bio-Rad); pre-stained SDS-PAGE standards (Bio-Rad) were used as molecular weight markers. Proteins were transferred to Hybond-P polyvinylidene difluoride (PDVF; GE Healthcare Life Sciences, Piscataway, NJ, USA) membranes at 50 V for 1.5 hours at 4°C. Protein transfer was verified by staining the membrane with Ponceau S. Monoclonal anti-SB transposase antibody (MAB2798; R&D Systems, Minneapolis, MN, USA) was resuspended at a final concentration of 500 μg/mL and diluted 1:500 to probe the membranes blocked with Superblock (Pierce, Rockford, IL, USA) containing 0.05% (v/v) Tween 20. A secondary goat anti-mouse horseradish peroxidase-conjugated antibody was diluted to 1:10,000 and developed using the Chemiluminescent Detection System (Pierce). Membranes were stripped with Restore Western Blot Stripping Solution (Pierce) and re-probed with a mouse monoclonal antibody specific for Xenopus β-actin (ab8224; Abcam, Cambridge, MA, USA) as a control for protein recovery.
Fluorescent protein expression analysis
A Leica FLIII fluorescent dissecting microscope was used to analyze GFP and RFP expression. Digital images were captured using a Nikon Ri1 color digital camera and the Nikon Elements Basic Research software package (Nikon, Melville, NY, USA). Tadpoles were immobilized for photography by brief anesthesia in 0.015% (w/v) tricaine methanesulphonate.
Southern blot hybridization
Genomic DNA was harvested from individual tadpoles by overnight proteinase K digestion at 56°C and phenol/chloroform/isoamyl alcohol extraction using standard protocols . Genomic DNA (3 μg to 5 μg) was digested with Bgl II, separated on a 0.7% (w/v) agarose gel and transferred to Hybond N+ hybridization transfer membranes (GE Healthcare Life Sciences, Piscataway, NJ, USA). The hybridization membranes were probed with a 32P-radiolabeled fragment of the GFP open reading frame (approximately 700 bp) and exposed onto a GE Healthcare Life Sciences phosphorimager screen for detection.
Genomic PCR and transposon integration site analysis
Integration site analysis was performed EPTS LM-PCR to the right arm (IR/DR) of the SB transposon as described previously [6, 8]. The integration sites were verified using genomic PCR strategies with primers that bind to scaffold sequences beyond the EPTS LM-PCR products. PCR primers were also designed to amplify the predicted sequences that flank the left IR/DR of the SB transposon. All genomic PCR products were cloned into either pGEM-T Easy (Promega) or TOPO-TA (Invitrogen, Carlsbad, CA, USA) and sequenced. Novel sequences were queried against the Joint Genome Institute X. tropicalis genome (version 4.1; http://genome.jgi-psf.org/Xentr4/Xentr4.home.html) and scaffolds were assigned to chromosomes and linkage groups based on the genetic map developed at the University of Houston .
List of abbreviations
chicken β-actin promoter coupled with a cytomegalovirus enhancer
- EPTS LM-PCR:
extension primer tag selection linker-mediated polymerase chain reaction
fluorescence in situ hybridization
green fluorescent protein
human chorionic gonadotropin
indirect repeat/direct repeats
polymerase chain reaction
red fluorescent protein
We thank Cheryl Winter for animal husbandry, Drs David Largaespada and Robert Grainger for providing plasmids (pCAGGS-SB 10 and 2.2 γ1-crystallin-RFP, respectively). We thank the following St. Jude Children's Research Hospital shared resources: the Hartwell Center of Bioinformatics and Biotechnology for DNA sequencing and bioinformatics support; the Cytogenetics Lab for FISH analysis; and the Animal Resource Center. Support for this study was provided by the National Institutes of Health (HD042994, MH079381 to PEM and HD046661 to AKS and DEW) and by the American Lebanese and Syrian Associated Charities (PEM).
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