Figure 2From: Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalisBreeding strategy to generate double-transgenic hopper frogs. The F2 hopper frogs were outcrossed with wild-type animals and the progeny was scored for GFP and RFP expression. The GFP-positive/RFP-negative F3 progeny were either raised to adulthood for outcross or genomic DNA was harvested after stage 45 for molecular analyses. GFP: green fluorescent protein; RFP: red fluorescent protein.Back to article page