Figure 6

Ty1 complementary (c)DNA levels in wild type and mutant yeast strains. Total genomic DNA was isolated from each strain, digested with Sph I, transferred to nitrocellulose and probed for Pol sequences. (A) Diagram and expected size (in kbp) of Ty1 sequences detected by the Pol probe (modified with permission [31]). Sph I sites are indicated by thin vertical lines and regions hybridizing to the Pol probe are shown as black rectangles. Ty1 LTR sequences are represented as black triangles; the hatched box on the plasmid represents the galactose-inducible promoter.(B) Autoradiogram of the Southern blot. Strains and temperature of galactose induction (°C) are indicated below the blot. The locations of the pGTy1 and Ty1 sequences detected by the Pol probe are indicated on the right; the locations of molecular size standards are indicated on the left. The endogenous Ty1 cDNA signal in each lane was normalized to the genomic bands G1 and G2. The ratio of cDNA signal relative to 30°C within each strain and the ratio of cDNA relative to wild type between strains is given in table 1. Strains are as follows: wild type JKc1356; rfx1Δ JKc1427; sml1Δ JKc1429.